NUCC-390

NUCC-390 is a novel and selective small-molecule CXCR4 receptor agonist. NUCC-390 can induce internalization of CXCR4 receptors[1].

Research Areas >> Others

Signaling Pathways >> GPCR/G Protein >> CXCR

Signaling Pathways >> Immunology/Inflammation >> CXCR

CXCR4

NUCC-390 (10 μM) produces strong (Ca)i response, but this effect can be blocked by the known potent and selective CXCR4 antagonist AMD3100[1]. NUCC-390 (10 μM) leads to increased levels of pERK, it has the capability of stimulating signaling activity downstream of CXCR4 receptors[1]. NUCC-390 exhibited the ability to induce CXCR4 receptor internalization, we assessed the cellular localization of YFP-tagged CXCR4 receptors expressed in HEK293 cells following treatment with SDF-1 or NUCC-390[1]. NUCC-390 (10 μM; 2 hours) can induce CXCR4 receptor internalization, and non--treated cells exhibits some diffuse expression of CXCR4-YFP throughout the cytosol and clear expression in the cell membrane in HEK cells[1]. Western Blot Analysis[1] Cell Line: C8161 cells  Concentration: 10 μM Incubation Time: Result: Increased the level of pERK[1].

[1]. Mishra RK, et al. Discovery and characterization of novel small-molecule CXCR4 receptor agonists and antagonists.Sci Rep. 2016 Jul 26;6:30155.