Fura-2, AM

Names

[ Name ]:
Fura-2, AM

[Synonym ]:
Fura 2am
Fura-2 pentakis(acetoxymethyl) ester
MFCD00036976
Acetoxymethyl 2-(6-{bis[2-(acetoxymethoxy)-2-oxoethyl]amino}-5-[2-(2-{bis[2-(acetoxymethoxy)-2-oxoethyl]amino}-5-methylphenoxy)ethoxy]-1-benzofuran-2-yl)-1,3-oxazole-5-carboxylate
Fura-2 AM

Biological Activity

[Description]:

Fura-2 AM is a ratiometric fluorescent Ca2+ indicator.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

[In Vitro]

The trypsin (100 nM) mediated Ca2+ transients are imagined in tsA-201 cells loaded with either 750 nM (n=111), 500 nM (n=119) or 250 nM (n=130) Fura-2 AM and compare the average Ca2+ increase above the baseline to the value obtained in the initial experiments using 1 μM of Fura-2 AM. The Ca2+ increases above the baseline for the 750, 500 and 250 nM are 633.0±33.9 nM (n=111, P>0.05 compare to 1 μM Fura-2 AM), 552.6±22.7 nM (n=119, P>0.05 compare to 1 μM Fura-2 AM) and 616.0±24.9 nM (n=130, P>0.05 compare to 1 μM Fura-2 AM), respectively[1].

[Cell Assay]

Cells are loaded with Fura-2 AM, which diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid. Aliquot 2-mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 µL of Fura-2 AM stock to generate a 1 µM Fura-2 AM solution. Vortex the solution vigorously for 1 minute. Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish. Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Prepare a 35 mm dish containing 2-mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish. Mount the coverslip on the imaging chamber[2].

[References]

[1]. Tinning PW, et al. A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision. J Microsc. 2017 Aug 24.

[2]. Odmara L Barreto-Chang, et al. Calcium Imaging of Cortical Neurons using Fura-2 AM. J Vis Exp. 2009 Jan 19;(23). pii: 1067.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.4±0.1 g/cm3

[ Boiling Point ]:
975.9±75.0 °C at 760 mmHg

[ Molecular Formula ]:
C₄₄H₄₇N₃O₂₄

[ Molecular Weight ]:
1001.850

[ Flash Point ]:
544.0±37.1 °C

[ Exact Mass ]:
1001.254944

[ PSA ]:
327.11000

[ LogP ]:
2.90

[ Vapour Pressure ]:
0.0±0.3 mmHg at 25°C

[ Index of Refraction ]:
1.567

[ Storage condition ]:
−20°C

[ Water Solubility ]:
DMSO: soluble

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

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