H-Gly-Arg-Gly-Asp-Ser-Pro-Lys-OH acetate salt

Names

[ CAS No. ]:
111119-28-9

[ Name ]:
H-Gly-Arg-Gly-Asp-Ser-Pro-Lys-OH acetate salt

[Synonym ]:
Grgdspk
Gly-arg-gly-asp-ser-pro-lys

Biological Activity

[Description]:

GRGDSPK is an inhibitory peptide for RGD-mediated adhesion between integrin and extracellular matrix molecules.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Research Areas >> Others

Peptides

[In Vitro]

GRGDSPK inhibits the adhesion of Rat PDL cells[1]. RGD-containing (GRGDSPK, 1.5 mM, 1.0 mM, and 0.5 mM) and RGD-mod-ified peptides impair the ability of sperm to fertilizebovine oocytes, illustrated by a reduction in cleavage in a dose-dependent manner[2].

[Cell Assay]

Proliferation assays are carried out in 48-well cell culture dishes coated with OCP or HL. In proliferation assays, 7.25×103 cells in 0.5 mL of culture medium are seeded in a 48-well dish. Culture medium is changed every 3 days. After 1, 6, 12, and 18 days of culture, plates are washed thrice with PBS to remove unattached cells, and attached cells are collected by trypsinization. The number of cells is counted using a hemocytometer.

[References]

[1]. Hatakeyama J, et al. Proliferation and adhesion of periodontal ligament cells on synthetic biominerals. Oral Dis. 2007 Sep;13(5):500-6.

[2]. Sessions BR, et al. Effects of amino acid substitutions in and around the arginine-glycine-aspartic acid (RGD) sequence on fertilization and parthenogenetic development in mature bovine oocytes. Mol Reprod Dev. 2006 May;73(5):651-7.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.59 g/cm3

[ Molecular Formula ]:
C₂₈H₄₉N₁₁O₁₁

[ Molecular Weight ]:
715.75600

[ Exact Mass ]:
715.36100

[ PSA ]:
374.58000

[ Index of Refraction ]:
1.672

[ Storage condition ]:
2-8℃

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ RIDADR ]:
NONH for all modes of transport

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Chemokine and integrin receptors must work in concert when circulating leukocytes mobilize toward a site of tissue inflammation or infection. In a previous study, we reported that ligation of the alph...