Tris-NTA

Names

[ CAS No. ]:
1146592-99-5

[ Name ]:
Tris-NTA

Biological Activity

[Description]:

Tris-NTA is a His-tagged protein ligand, which can be used to bind His-tagged proteins[1][2].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

Tris-NTA, which in presence of Ni2+ ions can interact with a His10-tag fused to the intracellular C terminus of the receptor. Whereas the Tris-NTA group shows an affinity Kd to bind the His10-tag of ~10-30 nM[1].

[References]

[1]. Moritz Pfreundschuh, et al. Identifying and quantifying two ligand-binding sites while imaging native human membrane receptors by AFM. Nat Commun. 2015 Nov 12;6:8857.

[2]. Luyao Liu, et al. Rapid and regenerable surface plasmon resonance determinations of biomarker concentration and biomolecular interaction based on tris-nitrilotriacetic acid chips. Anal Chim Acta. 2021 Jul 25;1170:338625.

Chemical & Physical Properties

[ Molecular Formula ]:
C₄₃H₆₈N₈O₂₂

[ Molecular Weight ]:
1049.04

Tris-NTA

Names

Biological Activity

Chemical & Physical Properties

Related Compounds