MCLA hydrochloride

Names

[ Name ]:
MCLA hydrochloride

[Synonym ]:
2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one Hydrochloride
6-(4-Methoxyphenyl)-2-methyl-3,7-dihydroimidazo[1,2-a]pyrazin-3(7H)-one hydrochloride
6-(4-methoxyphenyl)-2-methyl-7H-imidazo[1,2-a]pyrazin-3-one,hydrochloride
MFCD00144728
6-(4-Methoxyphenyl)-2-methylimidazo[1,2-a]pyrazin-3(7H)-one hydrochloride (1:1)
Imidazo[1,2-a]pyrazin-3(7H)-one, 6-(4-methoxyphenyl)-2-methyl-, hydrochloride (1:1)
6-(4-Methoxyphenyl)-2-methyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one Hydrochloride
6-(4-Methoxyphenyl)-2-methylimidazo[1,2-a]pyrazin-3(7H)-one hydrochloride
MCLA hydrochloride

Biological Activity

[Description]:

MCLA hydrochloride is a chemiluminescent reagent which can be used to quantify aqueous concentrations of superoxide.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

[In Vitro]

The non-specific luminescence remains almost constant for 10 min after the addition of MCLA hydrochloride (MCLA) and is not significantly influenced by SOD. The MCLA hydrochloride method is, however, 4.5-times more sensitive than the CLA method[1].

[Kinase Assay]

The standard reaction mixture contains 1×l0-7 M MCLA hydrochloride (MCLA), 5×10-5 M hypoxanthine, XOD (6.5 U), SOD (0.2 to 20 ng/mL) or none, and 50 mM Tris-HCl buffer containing 0.1 mM EDTA at pH 7.8, in a total volume of 3.0 mL. Chemiluminescence measurement is initiated by the addition of MCLA hydrochloride to the standard incubation mixture excluding XOD, continued for 4 min without additive and for an additional 4 min after the addition of XOD. Chemiluminescence is measured using a luminescence reader at 25°C[1].

[References]

[1]. Kimura H, et al. Highly sensitive and reliable chemiluminescence method for the assay of superoxide dismutase inhuman erythrocytes. FEBS Lett. 1988 Nov 7;239(2):347-50.

[Related Small Molecules]

Chemical & Physical Properties

[ Boiling Point ]:
410.3ºC at 760 mmHg

[ Molecular Formula ]:
C₁₄H₁₄ClN₃O₂

[ Molecular Weight ]:
291.733

[ Flash Point ]:
201.9ºC

[ Exact Mass ]:
291.077454

[ PSA ]:
59.39000

[ LogP ]:
2.80860

[ Vapour Pressure ]:
3.95E-07mmHg at 25°C

[ Storage condition ]:
2~8°C

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
F+

[ Safety Phrases ]:
S22

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3.0

[ HS Code ]:
2933990090

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Customs

[ HS Code ]: 2933990090

[ Summary ]:
2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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