Kaurane-3,16,17-triol

ent-Kaurane-3α,16β,17-triol (Compound 3) is an anticancer agent. ent-Kaurane-3α,16β,17-triol induces apoptosis in HCT116 cells[1].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

ent-Kaurane-3α,16β,17-triol (Compound 3; 0-100 μM; 24 h) 抑制 HepG2 和 HCT116 细胞增殖，IC50 值分别为 45.5 和 29.84 µM[1]。 ent-Kaurane-3α,16β,17-triol (20 and 30 μM; 24 h) 抑制 HCT116 细胞集落形成[1]。 ent-Kaurane-3α,16β,17-triol (30 and 40 µM; 48 h) 诱导人结肠癌细胞周期阻滞[1]。 ent-Kaurane-3α,16β,17-triol (30 and 40 µM; 72 h) 诱导人结肠癌细胞凋亡[1]。 Cell Viability Assay[1] Cell Line: HepG2, MDA-MB-231, SCG-7901, OVCAR3 and HCT116 cells Concentration: 0-100 μM Incubation Time: 24 h Result: Showed inhibitory effects with IC50s of 29.84, 45.5, >100, >100 and >100 μM against HCT116, HepG2, MDA-MB-231, SCG-7901 and OVCAR3 cells, respectively. Cell Cycle Analysis[1] Cell Line: HCT116 Concentration: 30 and 40 µM Incubation Time: 48 h Result: Significantly increased the cell population at the G0/G1 phase in a dosedependent manner. Apoptosis Analysis[1] Cell Line: HCT116 Concentration: 30 and 40 µM Incubation Time: 72 h Result: Increased the percentage of both early and late apoptotic cells. Western Blot Analysis[1] Cell Line: HCT116 Concentration: 30 μM Incubation Time: 72 h Result: Increased the expression levels of cleaved PARP, p27 and p53, and decreased the expression levels of cyclin D1 and CDK2.

[1]. Chen X, et al. Identification of terpenoids from Rubus corchorifolius L. f. leaves and their anti-proliferative effects on human cancer cells. Food Funct. 2017 Mar 22;8(3):1052-1060.