Lumiprobe CY 5 hydrazide hydrochloride

Lumiprobe CY 5 hydrazide hydrochloride (Cy5-hydrazide hydrochloride) is a fluorescent dye for aldehydes and ketones. Lumiprobe CY 5 hydrazide hydrochloride can be used for labeling proteins[1].

Research Areas >> Others

Signaling Pathways >> Others >> Others

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Cell viability assay[1]: 1. Add Lumiprobe CY 5 hydrazide hydrochloride to each sample at 25℃ for 1 h to label sample. 2. Remove salts and unbound fluorescent dye by the trichloroacetic acid (TCA esodium deoxycholate (DOC) or acetone method. 3. Measure fluorescence intensity (Ex=640 nm, Em=660 nm).

[1]. Yoshimura T, et al. A novel procedure for the assessment of the antioxidant capacity of food components. Anal Biochem. 2016 Aug 15;507:7-12.