Zy3 carboxylic acid

Zy3 carboxylic acid (Compound 7c) is a novel cyanine dyes with vinylsulfone group for labeling biomolecules with maximum wavelength (λabs) and emission maximum wavelength (λem) of 549 nm and 566 nm, respectively.

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

To ensure labeling efficiency, whether there is any effect from the difference in the length of the spacer is determine. For the 550 nm range, 7a, Zy3 carboxylic acid (Compound 7c), 7f, and 7g having five, six, eight, and eight carbon atoms in the spacer, respectively, are chosen. For the spacer-dependent reactivity test, dyes with the highest extinction coefficients (7g, 7l, and 7n) and those with ethyl groups as R on indole (7a, Zy3 carboxylic acid, 7f, and 7i) are selected. As a result, dyes with longer spacers are observed to have aster binding affinity to BSA. Among 7a, Zy3 carboxylic acid, 7f, and 7g, with the same absorption wavelength at ~550 nm (m=1)[1].

Stock solutions are prepared by dissolving bovine serum albumin (BSA, 10 mg/mL) in 0.1 M sodium phosphate buffer (pH 9.5) and 7a, Zy3 carboxylic acid (Compound 7c), 7f, 7g, 7i, 7l, and 7n (10 mg/mL) in distilled water. Each dye solution (7a (11.0 μL), Zy3 carboxylic acid (11.2 μL), 7f (11.6 μL), 7g (11.9 μL), 7i (11.7 μL), 7l (12.1 μL), and 7n (12.1 μL)) is reacted with the BSA solution (1 mL) in equivalent at 25 °C for 6 h. The labeling reactions are then monitored every 1 h via HPLC at 550, 650, and 750 nm (C6-Phenyl Column, 15-25% acetonitrile-water, 1.5 mL/min)[1].

[1]. Park JW, et al. Novel cyanine dyes with vinylsulfone group for labeling biomolecules. Bioconjug Chem. 2012 Mar 21;23(3):350-62.

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