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7-hydroxycoumarinyl-γ-Linolenate

Names

[ CAS No. ]:
161180-12-7

[ Name ]:
7-hydroxycoumarinyl-γ-Linolenate

[Synonym ]:
2-Oxo-2H-chromen-7-yl (6Z,9Z,12Z)-6,9,12-octadecatrienoate
6,9,12-Octadecatrienoic acid, 2-oxo-2H-1-benzopyran-7-yl ester, (6E,9E,12E)-
2-Oxo-2H-chromen-7-yl (6E,9E,12E)-6,9,12-octadecatrienoate
6,9,12-Octadecatrienoic acid, 2-oxo-2H-1-benzopyran-7-yl ester, (6Z,9Z,12Z)-
7-hydroxycoumarinyl--Linolenate

Biological Activity

[Description]:

7-Hydroxycoumarinyl-γ-linolenate is a cytosolic phospholipase A2 (cPLA2) fluorogenic substrate. 7-Hydroxycoumarinyl-γ-linolenate can be used to monitor the enzymatic activity of cPLA2[1].

[Related Catalog]:

Signaling Pathways >> Metabolic Enzyme/Protease >> Phospholipase
Research Areas >> Others

[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). cPLA2α activity assay[1][2]: 1. Sonicate 200 μL of 1,2-O-tetradecyl-sn-glycero-3-phosphocholine (DTPC)/Triton X-100 high-affinity mixed micelle solution containing 960 μM Triton X-100 (HY-Y1883A), 60 μM DTPC, 50 μM 7-hydroxycoumarinyl-γ-linolenate, 50 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (pH = 7.2), 0.3 mM EDTA (HY-Y0682), 1 mM CaCl2 and 300 mM KCl. 2. Monitoring the hydrolysis of 7-hydroxycoumarinyl-γ-linolenate continuously with excitation at 360 nm and emission at 460 nm. 3. The formation of 7-hydroxycoumarin was monitored by fluorospectrometer.

[References]

[1]. Xiao Q, et al. Bufexamac ameliorates LPS-induced acute lung injury in mice by targeting LTA4H. Sci Rep. 2016 Apr 29;6:25298.

[2]. Huang Z, et al. A continuous fluorescence-based assay for the human high-molecular-weight cytosolic phospholipase A2. Anal Biochem. 1994 Oct;222(1):110-5.

Chemical & Physical Properties

[ Density]:
1.1±0.1 g/cm3

[ Boiling Point ]:
563.5±50.0 °C at 760 mmHg

[ Molecular Formula ]:
C27H34O4

[ Molecular Weight ]:
422.556

[ Flash Point ]:
279.3±28.5 °C

[ Exact Mass ]:
422.245697

[ PSA ]:
56.51000

[ LogP ]:
7.58

[ Vapour Pressure ]:
0.0±1.5 mmHg at 25°C

[ Index of Refraction ]:
1.540

Related Compounds