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β-Amyloid (1-40), FAM-labeled

Names

[ CAS No. ]:
1678416-08-4

[ Name ]:
β-Amyloid (1-40), FAM-labeled

[Synonym ]:
2(1H)-Isoquinolineacetamide, N-(3-cyano-4,5,6,7-tetrahydrobenzo[b]thien-2-yl)-3,4-dihydro-
N-(3-Cyano-4,5,6,7-tetrahydro-1-benzothiophen-2-yl)-2-(3,4-dihydro-2(1H)-isoquinolinyl)acetamide

Biological Activity

[Description]:

β-Amyloid (1-40), FAM-labeled is a FAM fluorescently-labelled β-Amyloid (1-40) peptide (λex= 492 nm and λem= 518 nm)[1].

[Related Catalog]:

Signaling Pathways >> Others >> Others
Research Areas >> Neurological Disease

[Target]

λex= 492 nm; λem= 518 nm


[In Vitro]

Apical-to-basolateral exchange across endothelial monolayer of fluorescent Aβ42 (FAM-labeled Human β-Amyloid (1-42) and Fluor™488-labeled β-Amyloid (1-42)) or scramble Aβ42 (FAM-labeled scrambled β-Amyloid (1-42)) (1-100 μM) is monitored in transendothelial electrical resistance (TEER) during cell monolayer’s formation over time (over 120 min in presence or absence of Aβ24). Human β-Amyloid (1-24) (1 μM) results in H-Aβ42 retention, thus reducing its efflux through the BBB and therefore preventing an efficient mechanism of Aβ42 clearance[1]. The in vitro BBB model is used to investigate the passage of FAM-labeled or 488-conjugated H-Aβ42 across the endothelial cell monolayer. The addition of fluorescently labeled H-Aβ42 (FAM-labeled Human β-Amyloid (1-42) or Fluor™ 488-labeled β-Amyloid (1-42)) to the apical side of cell inserts results in effective BBB crossing, FAM-labeled scrambled β-Amyloid (1-42) is not efficiently transcytosed.

[References]

[1]. L M Jungbauer, et al. Preparation of fluorescently-labeled amyloid-beta peptide assemblies: the effect of fluorophore conjugation on structure and function. J Mol Recognit. Sep-Oct 2009;22(5):403-13.

[2]. Sonia Mazzitelli, et al. Amyloid-β 1-24 C-terminal truncated fragment promotes amyloid-β 1-42 aggregate formation in the healthy brain. Acta Neuropathol Commun. 2016 Oct 10;4(1):110.

Chemical & Physical Properties

[ Density]:
1.3±0.1 g/cm3

[ Boiling Point ]:
599.7±50.0 °C at 760 mmHg

[ Molecular Formula ]:
C20H21N3OS

[ Molecular Weight ]:
351.465

[ Flash Point ]:
316.5±30.1 °C

[ Exact Mass ]:
351.140533

[ LogP ]:
4.45

[ Vapour Pressure ]:
0.0±1.7 mmHg at 25°C

[ Index of Refraction ]:
1.661


Related Compounds