Ac-Asp-Glu-Val-Asp-AMC ammonium salt

Names

[ Name ]:
Ac-Asp-Glu-Val-Asp-AMC ammonium salt

[Synonym ]:
4-[(2-acetamido-3-carboxypropanoyl)amino]-5-[[1-[[3-carboxy-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopropan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-5-oxopentanoic acid
Ac-DEVD-AMC
MFCD00671411
L-α-Asparagine, N-acetyl-L-α-aspartyl-L-α-glutamyl-L-valyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-
N-Acetyl-L-α-aspartyl-L-α-glutamyl-L-valyl-N-(4-methyl-2-oxo-2H-chromen-7-yl)-L-α-asparagine

Biological Activity

[Description]:

Ac-DEVD-AMC is the Caspase-3 substrate. Sequence: N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-Methylcoumarin.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

Peptides

Research Areas >> Cancer

[In Vitro]

Ac-DEVD-AMC is the caspase-3 substrate and could be used to monitor intracellular caspase-3 activity[1].The AMC moiety is highest at 2 h (area =5.6±1.8), the AMC peak area for Ac-DEVD-AMC incubated without lung specimen (substrate alone) for 6 h is relatively small[2].

[Cell Assay]

Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2 : 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through microcentrifuge filter, separateed on HPLC, and analyzed for the fluorogenic AMC moiety[2].

[References]

[1]. Ahmed R Alsuwaidi, et al. Bioenergetics of murine lungs infected with respiratory syncytial virus. Virol J. 2013; 10: 22.

[2]. Ahmed R Alsuwaidi, et al. Lung tissue bioenergetics and caspase activity in rodents. BMC Res Notes. 2013; 6: 12.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.4±0.1 g/cm3

[ Boiling Point ]:
1200.1±65.0 °C at 760 mmHg

[ Molecular Formula ]:
C₃₀H₃₇N₅O₁₃

[ Molecular Weight ]:
675.641

[ Flash Point ]:
679.6±34.3 °C

[ Exact Mass ]:
675.238770

[ PSA ]:
287.61000

[ LogP ]:
1.07

[ Vapour Pressure ]:
0.0±0.3 mmHg at 25°C

[ Index of Refraction ]:
1.594

[ Storage condition ]:
−20°C

[ Water Solubility ]:
DMSO: 10 mM

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

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