4-Methylumbelliferyl Oleate

Names

[ Name ]:
4-Methylumbelliferyl Oleate

[Synonym ]:
4-MethylumbelliferylOleateA.R.
OLEIC ACID-4-METHYLUMBELLIFERYL ESTER
Einecs 242-210-7
4-Methylumbelliferone oleate
7-Hydroxy-4-MethylcouMarin Oleate
MFCD00076095
Oleic acid 4-methylumbelliferyl ester
4-methyl-2-oxo-2H-1-benzopyran-7-yl oleate
Oleoyl 4-MethyluMbelliferone

Biological Activity

[Description]:

4-Methylumbelliferyl oleate is a fluorogenic substrate for acid and alkaline lipases. 4-Methylumbelliferyl oleate is cleaved by lipases, liberating 4-Methylumbelliferyl (Ex/Em=320/450 nm)[1][2].

[Related Catalog]:

Signaling Pathways >> Others >> Others

Research Areas >> Metabolic Disease

[In Vitro]

The inhibitory activity against pancreatic lipase was measured using 4-Methylumbelliferyl (4-MU) oleate as a substrate. The reaction mixture consisted of 50 µL 0.1 mM 4-MU oleate, 20 µL McIlvane buffer (0.1 M citrate-Na2 HPO4, pH 7.4), and 5 µL of sample solution. Porcine pancreatic lipase (25 µL) was added to the reaction mixture and the final volume was adjusted to 0.1 ml. After the mixture was incubated at 37℃ for 10 min, the amount of 4-MU released by the lipase was measured using a fluorescence multi-detection reader at an excitation wavelength of 320 nm and an emission wavelength of 450 nm[1].

[References]

[1]. Zhang J, et al. Pancreatic lipase inhibitory activity of taraxacum officinale in vitro and in vivo. Nutr Res Pract. 2008 Winter;2(4):200-3.

[2]. Koster JF, et al. Study of the hydrolysis of 4-methylumbelliferyl oleate by acid lipase and cholesteryl oleate by acid cholesteryl esterase in human leucocytes, fibroblasts and liver. Biochim Biophys Acta. 1980 Apr 18;618(1):98-105.

Chemical & Physical Properties

[ Density]:
1.023g/cm3

[ Boiling Point ]:
571.5ºC at 760mmHg

[ Melting Point ]:
37-38ºC(lit.)

[ Molecular Formula ]:
C₂₈H₄₀O₄

[ Molecular Weight ]:
440.61500

[ Flash Point ]:
281.3ºC

[ Exact Mass ]:
440.29300

[ PSA ]:
56.51000

[ LogP ]:
8.04430

[ Vapour Pressure ]:
4.53E-13mmHg at 25°C

[ Index of Refraction ]:
1.515

[ Storage condition ]:
−20°C

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ HS Code ]:
2932209090

Synthetic Route

Click to get detail synthetic route

Customs

[ HS Code ]: 2932209090

[ Summary ]:
2932209090. other lactones. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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