st-Ht31

st-Ht31 is a membrane-permeable peptide inhibitor of protein kinase A (PKA) anchoring. st-Ht31 induces robust cholesterol/phospholipid efflux. st-Ht31 completely reverses foam cell formation and restores the metabolic health of macrophage[1][2].

Signaling Pathways >> Cytoskeleton >> PAK

Research Areas >> Metabolic Disease

Signaling Pathways >> Cell Cycle/DNA Damage >> PAK

st-Ht31 (5 µM; 2 h) 增强 BHK 细胞和 RAW 巨噬细胞中的胆固醇/磷脂流出[1]。 st-Ht31 (10µM; 24 h) 逆转泡沫细胞形成并恢复巨噬细胞的代谢健康[1]。st-Ht31 (20 µM; 20 min) 消除 HEK293T 细胞中 PMA 诱导的 ERK1/2 激活的 cAMP 抑制[2]。 Western Blot Analysis[2] Cell Line: HEK293T cells Concentration: 20 µM Incubation Time: 20 min Result: Abolished the cAMP inhibition of PMA-induced ERK1/2 activation.

[1]. Ma L, et al. Ht31, a protein kinase A anchoring inhibitor, induces robust cholesterol efflux and reverses macrophage foam cell formation through ATP-binding cassette transporter A1. J Biol Chem. 2011 Feb 4;286(5):3370-8.  

[2]. Rahamim Ben-Navi L, et al. A-Kinase Anchoring Protein 4 (AKAP4) is an ERK1/2 substrate and a switch molecule between cAMP/PKA and PKC/ERK1/2 in human spermatozoa. Sci Rep. 2016 Nov 30;6:37922.