Fura-FF pentapotassium

Fura-FF pentapotassium is a low-affinity fluorescent dye for calcium (Ex/Em: 365/514 nm in the absence of calcium; 339/507 nm in the presence of a high calcium concentration)[1].

Research Areas >> Others

Signaling Pathways >> Others >> Others

Guidelines[2] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Human Sperm Cells: 1. Incubate the cells according to your normal protocol. 2. Load cells with 5 μM Fura-FF in 1 mL Hepes-buffered human sperm (H-HSM) medium at 36 ℃ for 40 min. 3. Wash the sample by centrifugation and scatter cells in disposable centrifuge tubes at 1 ◊ 107 cells in 3 mL of H-HSM and maintained at 36 ℃. 4. Immediately measure fluorescence recordings.

[1]. Ruggiu AA, et al. Fura-2FF-based calcium indicator for protein labeling. Org Biomol Chem. 2010 Aug 7;8(15):3398-401.

[2]. Neri-Vidaurri Pdel C, et al. A remarkable increase in the pHi sensitivity of voltage-dependent calcium channels occurs in human sperm incubated in capacitating conditions. Biochem Biophys Res Commun. 2006 Apr 28;343(1):105-9.