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Orexin A human, rat, mouse

Names

[ CAS No. ]:
205640-90-0

[ Name ]:
Orexin A human, rat, mouse

[Synonym ]:
M.W. 3561.12 C152H243N47O44S4
Hypocretin 1
OREXINS OREXIN A HUMAN,RAT,MOUSE
Oroxin A
OREXIN A,BOVINE,HUMAN,MOUSE,RAT
Baicalin-7-glucoside
HYPOCRETIN
OREXIN A
PYR-PRO-LEU-PRO-ASP-CYS-CYS-ARG-GLN-LYS-THR-CYS-SER-CYS-ARG-LEU-TYR-GLU-LEU-LEU-HIS-GLY-ALA-GLY-ASN-HIS-ALA-ALA-GLY-ILE-LEU-THR-LEU-NH2(DISULFIDE BRIDGE: CYS6-CYS12,CYS7-CYS14)
Pyr-PLPDCCRQKTCSCRLYELLHGAGNHAAGILTL-NH2(Disulfidebridge:6-12and7-14)
Rat orexin A

Biological Activity

[Description]:

Rat orexin A is an important neuropeptide involved in the regulation of feeding, arousal, energy consuming, and reward seeking in the body.

[Related Catalog]:

Signaling Pathways >> Others >> Others
Research Areas >> Neurological Disease
Peptides

[In Vitro]

Rat orexin A is an important neuropeptide involved in the regulation of feeding, arousal, energy consuming, and reward seeking in the body. It is found that Rat orexin A promotes astrocytes migration in different time and concentrations. Rat orexin A has a maximal effect at 10 nM and 24 h treatment (increases the migrating distance of the astrocytes into ~240%, increases the migrating area of the astrocytes into ~190%). Results also demonstrate that Rat orexin A-induced phosphorylation of ERK1/2 is significantly elevated (0.38±0.03 in Rat orexin A 10 nM group vs. 0.21±0.01 in control group, n=3, p<0.05) in primary cortical astrocytes.

[In Vivo]

Rat orexin A delays the day of vaginal opening in rats of Group 4 comparing to that in Group 3. Results indicate that injection of Rat orexin A can ameliorate central precocious puberty in rats. Lower mRNA level of MEG3 is observed in precocious puberty rats injected with Rat orexin A than that in precocious puberty rats injected with normal saline. Rat orexin A also reverses the dysregulation of Kisspeptin in hypothalamus of central precocious puberty rats[2]. In A6, many or most dopamine β hydroxylase (DBH)-positive neurons appear to be activated by Rat orexin A. Results also demonstrate that food intake is significantly enhanced by local Rat orexin A injection (P<0.05; n=9)[3].

[Cell Assay]

Astrocyte cells are grown to confluence in dishes and starved with serum-free DMED/F12 medium for 24 h. The monolayer cells are manually scratched in the center of the dishes with a bright and clear field. The detached cells are removed by washing the cells once with PBS. Serum-free DMEM/F12 medium with or without Rat orexin A is added to each dish as indicated after pretreatment with the inhibitor for 30 min. Then the cells are separately fixed with 4% paraformaldehyde and labeled with anti-GFAP (1:200) for photography and analyzing of the cell migration at 0, 24 and 48 h. Images of migratory cells from the scratched boundary are observed and acquired at different time with a digital camera and a light microscope[1].

[Animal admin]

Five-days-old specific pathogen free grade female SD rats, weighing (25±5) g are used in this study. After two days of adaptive feeding, rats are randomly divided into four groups: Group 1 (normal control, n=12), Group 2 (precocious puberty rats, n=12), Group 3 (precocious puberty rats treated with normal saline, n=12), Group 4 (precocious puberty rats treated with Rat orexin A, n=12). Each rat in experimental groups 2, 3 and 4 are subcutaneously injected with 300 μg of danazol to establish the precocious puberty model. Each rat in groups 4 is injected with Rat orexin A (0.5 nM) for 1 time/day via lateral ventricle at 15-days-old. Each rat in groups 3 is injected with equal volumes of normal saline. When rats are 20-days-old, vaginal openings are checked and vaginal opening time (VO) is recorded[2].

[References]

[1]. Shu Q, et al. Orexin-A promotes cell migration in cultured rat astrocytes via Ca2+-dependent PKCα and ERK1/2 signals. PLoS One. 2014 Apr 18;9(4):e95259.

[2]. Tao YH, et al. Lateral ventricle injection of orexin-A ameliorates central precocious puberty in rat via inhibiting the expression of MEG3. Int J Clin Exp Pathol. 2015 Oct 1;8(10):12564-70.

[3]. Li AJ, et al. Orexin-A enhances feeding in male rats by activating hindbrain catecholamine neurons. Am J Physiol Regul Integr Comp Physiol. 2015 Aug 15;309(4):R358-67.


[Related Small Molecules]

Captisol | Cyclosporin A | H2DCFDA | 0MPTP hydrochloride | GW4869 | Etomoxir | TD139 | Mitoquinone mesylate | GSK2795039 | JC-1 | BAPTA-AM | AP 20187 | Setanaxib (GKT137831) | D-Luciferin | Crotaline

Chemical & Physical Properties

[ Molecular Formula ]:
C152H243N47O44S4

[ Molecular Weight ]:
3561.10

[ PSA ]:
1589.89000

[ LogP ]:
1.15650

Related Compounds