Ned-K

Ned-K is a nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist. Ned-K is effective at dampening simulated ischaemia and reperfusion (sIR)-induced Ca2+ oscillations in cardiomyocytes[1].

Signaling Pathways >> Membrane Transporter/Ion Channel >> Calcium Channel

Research Areas >> Cardiovascular Disease

Ned-K suppresses Ca2+ oscillations and dramatically protects cardiomyocytes from cell death in vitro after ischaemia and reoxygenation, preventing opening of the mitochondrial permeability transition pore. Ned-K (10 µM) almost completely eliminates [Ca2+]c oscillations, and Ned-K (0.1 µM) is effective at suppressing [Ca2+]c levels[1]. Cell Viability Assay[1] Cell Line: Dead primary adult cardiomyocytes Concentration: 0.1 and 10 µM Incubation Time: Result: Treatment with 0.1 µM caused a slight decrease in cardiomyocyte death (34±6%). Treatment with 10 μM at reoxygenation significantly decreased cell death after sIR to 16±1%.

Injection of Ned-K causes a significant reduction in infarct size in mice. Ned-K (administered i.v. to mice 5 min before reperfusion) significantly decreases myocardial infarct size relative to area at risk[1].

[1]. Sean M Davidson, et al. Inhibition of NAADP signalling on reperfusion protects the heart by preventing lethal calcium oscillations via two-pore channel 1 and opening of the mitochondrial permeability transition pore. Cardiovasc Res. 2015 Dec 1;108(3):357-66.