Influenza A virus-IN-1

Influenza A virus-IN-1 is a dihydropyrrolidones derivative and is a potent inhibitor against wide subtypes of influenza A virus (IAV) with IC50 values from 3.11 μM to 7.13 μM. Influenza A virus-IN-1 efficiently inhibits replication of IAV, up-regulates the expression of key antiviral cytokines IFN-β and antiviral protein MxA[1].

Signaling Pathways >> Anti-infection >> Influenza Virus

Research Areas >> Infection

IC50: 3.11 μM (A/Puerto Rico/8/34 (H1N1)), 3.58 μM (A/FM-1/1/47 (H1N1)), 5.26 μM (A/Aichi/2/68 (H3N2)), 6.48 μM (A/PR/8/34 (H1N1) with NA-H274Y) and 7.13 μM (The influenza A virus 690 (H3))

Influenza A virus-IN-1 (compound 5-2; 5-40 μM; 24 hours; MDCK cells) treatment significantly decreases the yields of influenza viral NP in a dose-dependent manner. And the expression level of NP protein could also be restrained in a dose-dependent manner[1]. Influenza A virus-IN-1 (compound 5-2) treatment reduces influenza HA mRNA expression at all three time points[1]. Influenza A virus-IN-1 (compound 5-2) could efficiently inhibit replication of IAV and suppress the production of the NDAPH oxidase NOX1 in MDCK cells[1]. Western Blot Analysis[1] Cell Line: IAV-infected MDCK cells Concentration: 5 μM, 10 μM, 20 μM, 40 μM Incubation Time: 24 hours Result: Significantly decreased the yields of influenza viral NP in a dose-dependent manner.

[1]. Teng Liu, et al. Discovery of Dihydropyrrolidones as Novel Inhibitors Against Influenza A Virus. Eur J Med Chem. 2020 Aug 1;199:112334.