5-epi-Arvestonate A

5-epi-Arvestonate A is a sesquiterpenoid isolated from the whole plants of Seriphidium transiliense. 5-epi-Arvestonate A promotes melanogenic production by activating the transcription of microphthalmia-associated transcription factor (MITF) and tyrosinase family genes. 5-epi-Arvestonate A inhibits the expression of IFN-γ-chemokine through the JAK/STAT signaling pathway in immortalized human keratinocyte (HaCaT) cells[1].

Research Areas >> Cancer

Signaling Pathways >> Metabolic Enzyme/Protease >> Tyrosinase

5-epi-Arvestonate A (50 μM; 48 h) activates the transcription of microphthalmia-associated transcription factor (MITF) and tyrosinase family genes in murine melanoma (B16) cells[1]. 5-epi-Arvestonate A (50 μM; 48 h) increases both the mRNA and protein level of TRP-1, TRP-2, TYR, and MITF in murine melanoma (B16) cells[1]. 5-epi-Arvestonate A (10 μM, 50 μM; 24 h) disrupts the IFN-γ-chemokine and CXCL10/9 production through the JAK/STAT signal pathway in IFN-γ-activated HaCaT cells[1]. Western Blot Analysis[1] Cell Line: Murine melanoma (B16) cells; IFN-γ-activated HaCaT cells Concentration: 1, 10, 50 μM Incubation Time: 24 hours and 48 hours Result: Increased the protein level of TRP-1, TRP-2, TYR, and MITF at 50 μM concentration in murine melanoma (B16) cells. Decreased the protein level of IFN-γ, CXCL9/10 in IFN-γ-activated HaCaT cells for 24 hr. Cell Viability Assay[1] Cell Line: Murine melanoma (B16) cells Concentration: 1, 10, 50 μM Incubation Time: 48 hours Result: Increased the viability of B16, increased the levels of both relative melanin content and relative tyrosinase activity.