2,3,5-Triphenyltetrazolium chloride

Names

[ CAS No. ]:
298-96-4

[ Name ]:
2,3,5-Triphenyltetrazolium chloride

[Synonym ]:
2,3,5-triphenyl tetrazolium chloride
TETRAZOLE RED
TTC
Red tetrazolium
triphenyltetrazolium chloride
urocheck
TPTZ,TTZ,Tetrazolium Red
TTL
EINECS 206-071-6
vitastain
2,3,5-Triphenyltetrazolium chloride
2,3,5-Triphenyl-2H-tetrazol-3-ium chloride
tetrazolium red
2H-Tetrazolium, 2,3,5-triphenyl-, chloride (1:1)
MFCD00011963
2,3,5-tetraphenyltetrazolium chloride
uroscreen
2,3,5-triphenyl-2H-tetrazolium chloride
TTZ

Biological Activity

[Description]:

Tetrazolium Red(2,3,5-Triphenyltetrazolium chloride; TPTZ) is used to visualize dehydrogenase enzyme activity; initially the tetrazolium solution is colorless but changes to red when it comes into contact with hydrogen. Tetrazolium red is used in a biochemical viability test for seeds. The test relies on dehydrogenase enzymes to release hydrogen ions which subsequently reduce the colorless tetrazolium salt solution to a red compound called formazan. Living cells turn red while dead cells remain colorless.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Biochemical Assay Reagents

Research Areas >> Others

[Related Small Molecules]

Chemical & Physical Properties

[ Boiling Point ]:
>360

[ Melting Point ]:
250 °C(lit.)

[ Molecular Formula ]:
C₁₉H₁₅ClN₄

[ Molecular Weight ]:
334.802

[ Flash Point ]:
91.7ºC

[ Exact Mass ]:
334.098511

[ PSA ]:
34.59000

[ LogP ]:
0.21500

[ Vapour Pressure ]:
0.0615mmHg at 25°C

[ Storage condition ]:
2-8°C

[ Stability ]:
Stable. Light sensitive. Protect from moisture. Incompatible with strong oxidizing agents.

[ Water Solubility ]:
H2O: may be clear to slightly hazy

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: XF8100000

CHEMICAL NAME :: 2H-Tetrazolium, 2,3,5-triphenyl-, chloride

CAS REGISTRY NUMBER :: 298-96-4

LAST UPDATED :: 199701

DATA ITEMS CITED :: 4

MOLECULAR FORMULA :: C19-H15-N4.Cl

MOLECULAR WEIGHT :: 334.83

WISWESSER LINE NOTATION :: T5NNKNJ BR& CR& ER &G

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 5600 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: CSLNX* U.S. Army Armament Research & Development Command, Chemical Systems Laboratory, NIOSH Exchange Chemicals. (Aberdeen Proving Ground, MD 21010) Volume(issue)/page/year: NX#00925 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - 83592 No. of Facilities: 11 (estimated) No. of Industries: 1 No. of Occupations: 1 No. of Employees: 32 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 83592 No. of Facilities: 32 (estimated) No. of Industries: 1 No. of Occupations: 2 No. of Employees: 1665 (estimated) No. of Female Employees: 1207 (estimated)

Safety Information

[ Symbol ]:

GHS07

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H315-H319-H335

[ Precautionary Statements ]:
P261-P305 + P351 + P338

[ Personal Protective Equipment ]:
Eyeshields;Gloves

[ Hazard Codes ]:
Xi:Irritant

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
S26-S36

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ RTECS ]:
XF8100000

[ HS Code ]:
2933990090

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Customs

[ HS Code ]: 2933990090

[ Summary ]:
2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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