4-Nitrophenyl phosphate disodium hexahydrate

Names

[ Name ]:
4-Nitrophenyl phosphate disodium hexahydrate

[Synonym ]:
MFCD00066288
di-Sodium 4-nitrophenyl phosphate
pNPP disodium salt hexahydrate
DisodiuM 4-Nitrophenylphosphate Hexahydrate
Phosphoric Acid 4-Nitrophenyl Ester Disodium Salt Hexahydrate
Phosphatase substrate
4-Nitrophenyl Phosphate,Disodium Salt Hexahydrate
p-Nitrophenyl phosphate disodium hexahydrate
pNPP disodium hexahydrate
4-Nitrophenyl phosphate disodium salt hexahydrate
p-Nitrophenyl phosphate disodium hexahydrate,Disodium 4-nitrophenyl phosphate hexahydrate,di
Sodium 4-nitrophenyl phosphate hexahydrate
EINECS 224-246-5
Disodium 4-Nitrophenyl Phosphate Hexahydrate

Biological Activity

[Description]:

4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

4-Nitrophenyl phosphate (PNPP) disodium hexahydrate is a commonly used substrate for alkaline phosphatases (ALPs). 4-Nitrophenyl phosphate disodium hexahydrate is hydrolyzed by ALP to PNP (p-nitrophenol), which quenches the fluorescence of novel gold nanoclusters (AuNCs) by the inner filter effect (IFE)[2].

[References]

[1]. Lountos GT, et al. Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate. Acta Crystallogr F Struct Biol Commun. 2015;71(Pt 2):199-205.

[2]. Qi S, et al. Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect. Analyst. 2020;145(11):3871-3877.

Chemical & Physical Properties

[ Boiling Point ]:
457.8ºC at 760mmHg

[ Melting Point ]:
>300ºC(lit.)

[ Molecular Formula ]:
C₆H₁₆NNa₂O₁₂P

[ Molecular Weight ]:
371.14400

[ Flash Point ]:
230.7ºC

[ Exact Mass ]:
371.02100

[ PSA ]:
183.43000

[ LogP ]:
2.08010

[ Appearance of Characters ]:
tablets | white to pale yellow

[ Storage condition ]:
2-8°C

[ Water Solubility ]:
H2O: 25 mg/mL at 20 °C, clear, yellow-green

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Safety Phrases ]:
24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

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