ROS Probe, HPF

Hydroxyphenyl fluorescein (HPF) is the reagent that can directly detect highly reactive oxygen species (hROS). Hydroxyphenyl fluorescein selectively and dose-dependently reacts with hROS, such as the hydroxyl radical and peroxynitrite, which exhibit strong fluorescence[1][2].

Research Areas >> Others

The fluorescence of Hydroxyphenyl Fluorescein (HPF) is greatly enhanced following incubation with blue light illuminated blebbistatin (30 min). Fluorescence enhancement of Hydroxyphenyl Fluorescein occurred equally efficiently under Ar purged and non-Ar purged conditions, suggesting that singlet oxygen as an intermediate involved is improbable. Fluorescence measurement with Hydroxyphenyl Fluorescein suggests a negative linear relationship between fluorescence intensity of Hydroxyphenyl Fluorescein (10 μM) and thiourea concentration (0-1 mM)[1].

The Hydroxyphenyl Fluorescein (HPF) probe (15 μl, 25 μM) is given intratesticularly in anaesthetized mice 20 min before IR (ionizing radiation). The accumulation of •OH by the fluorescence signal emitted by the oxidized form of HPF is assessed[3].

[1]. Ming-De Li, et al. Dynamics of Oxygen-Independent Photocleavage of Blebbistatin as a One-Photon Blue or Two-Photon Near-Infrared Light-Gated Hydroxyl Radical Photocage. J Am Chem Soc. 2018 Nov 21;140(46):15957-15968.

[2]. Yoichiro Masuda, et al. Free radical production by femtosecond laser lens irradiation in porcine eyes. J Cataract Refract Surg. 2019 Aug;45(8):1168-1171.

[3]. Yunhai Chuai, et al. Hydrogen-rich saline attenuates radiation-induced male germ cell loss in mice through reducing hydroxyl radicals. Biochem J. 2012 Feb 15;442(1):49-56.