NS3694

[Description]:

NS3694, a diarylurea compound, is an apoptosome inhibitor. NS3694 inhibits apoptosome formation and caspase activation[1].

[Related Catalog]:

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

[In Vitro]

When cell extracts are stimulated with cytochrome c and dATP in the presence of NS3694 (10-100 μM; 120 min), a dose-dependent inhibition of caspase 9, 3, and 7 processing, DFF45/ICAD and PARP cleavage, and DEVDase activity is observed[1]. NS3694 (10-100 μM) inhibits TNF-induced effector caspase activation and apoptosis in MCF-casp3 cells[1]. NS3694 specifically suppresses the cytochrome C-induced formation of apoptosome complex and caspase-9 activation, thus inhibiting the mitochondria-mediated apoptosis. NS3694 inhibits the formation of the apoptosome Apaf-1 by blocking the activation of the initiator caspase-9[1]. Western Blot Analysis[1] Cell Line: HeLa cells Concentration: 10 μM, 50 μM, 100 μM Incubation Time: 120 min Result: Inhibited cytochrome c- and dATP-induced DEVDase activation and processing of caspases and caspase substrates.

[References]

[1]. Ulrik Lademann, et al. Diarylurea compounds inhibit caspase activation by preventing the formation of the active 700-kilodalton apoptosome complex. Mol Cell Biol. 2003 Nov;23(21):7829-37.

[ Density]:
1.574g/cm3

[ Boiling Point ]:
378.448ºC at 760 mmHg

[ Molecular Formula ]:
C₁₅H₁₀ClF₃N₂O₃

[ Molecular Weight ]:
358.70000

[ Flash Point ]:
182.679ºC

[ Exact Mass ]:
358.03300

[ PSA ]:
78.43000

[ LogP ]:
4.84700

[ Index of Refraction ]:
1.638

[ Storage condition ]:
-20°C

Click to get detail MSDS

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ RIDADR ]:
NONH for all modes of transport

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