Benzenesulfonic acid,5-chloro-3-[2-(2,4-dihydroxyphenyl)diazenyl]-2-hydroxy-

Names

[ Name ]:
Benzenesulfonic acid,5-chloro-3-[2-(2,4-dihydroxyphenyl)diazenyl]-2-hydroxy-

[Synonym ]:
2,2',4'-TRIHYDROXY-5-CHLOROAZOBENZENE-3-SULFONIC ACID
LUMOGALLION,FLUORIMETRIC REAGENT FOR AL,GA AND OTHER METALS
Lumohallion IREA
LuMogallion ,Ga and other Metals]
2-hydroxy-3-sulfo-5-chlorobenzenazoresorcinol
LUMOGALLION
4-Chloro-6-(2,4-dihydroxyphenylazo)-1-phenol-2-sulfonic Acid
2,2',4'-Trihydroxy-5-chloroazobenzene-3-sulfonic Acid
2,2,4-Trihydroxy-5-Chloroazobenzene-3-Sulfonic Acid

Biological Activity

[Description]:

Lumogallion is a highly sensitive fluorescent reagent for the detection of aluminum, gallium and other metals. Lumogallion has an excitation wavelength of 490 nm and an emission spectrum in the range of 520 nm to 650 nm, with a peak near 580 nm[1][2].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

Lumogallion (1 mM, 45 min) can be used to detect aluminum levels in brain tissue of Alzheimer's patients[1]. Lumogallion can be used as a probe to stain live cells with intracellular aluminum adjuvant[2]. Lumogallion (10 μM, 60 min) can be used to detect aluminum content in root tip cell tissue of plants[3]. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Dissolved lumogallion in 0.1 M acetate buffer (pH 5.2). 2. Sample and 10 μM lumogallion were co-incubated for 60 min in the dark. 3. Washed the mixed sample well with acetate buffer solution twice, 15 minutes each, and observed under a confocal laser microscope. 4. Excitation wavelength is 488 nm , emission wavelength is 520 nm.

[References]

[1]. Ambreen Mirza, et al. The Identification of Aluminum in Human Brain Tissue Using Lumogallion and Fluorescence Microscopy. J Alzheimers Dis. 2016 Oct 18;54(4):1333-1338.

[2]. Irene Mile, et al. Al adjuvants can be tracked in viable cells by lumogallion staining. J Immunol Methods. 2015 Jul;422:87-94.

[3]. Tatsuhiko Kataoka, et al. Highly sensitive analytical method for aluminum movement in soybean root through lumogallion staining. J. Plant Res. 110, 305–309 (1997).

Chemical & Physical Properties

[ Density]:
1.75g/cm3

[ Molecular Formula ]:
C₁₂H₉ClN₂O₆S

[ Molecular Weight ]:
344.72800

[ Exact Mass ]:
343.98700

[ PSA ]:
148.16000

[ LogP ]:
4.19970

[ Index of Refraction ]:
1.706

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: DB5300000

CHEMICAL NAME :: Benzenesulfonic acid, 5-chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxy-

CAS REGISTRY NUMBER :: 4386-25-8

LAST UPDATED :: 199712

DATA ITEMS CITED :: 1

MOLECULAR FORMULA :: C12-H9-Cl-N2-O6-S

MOLECULAR WEIGHT :: 344.74

WISWESSER LINE NOTATION :: WSQR BQ EG CNUNR BQ DQ

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 180 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: CSLNX* U.S. Army Armament Research & Development Command, Chemical Systems Laboratory, NIOSH Exchange Chemicals. (Aberdeen Proving Ground, MD 21010) Volume(issue)/page/year: NX#04790

Safety Information

[ Risk Phrases ]:
20/21/22

[ Safety Phrases ]:
26-36/37/39