ODN 2007

ODN 2007, a class B CpG ODN (oligodeoxynucleotide), is a Toll-like receptor (TLR) ligand. ODN 2007 can be used as an immunomodulator, vaccine adjuvant, and enhance immune responses in mammals, fish, and humans. ODN 2007 sequence: 5'-TCGTCGTTGTCGTTTTGTCGTT-3'[1][2][3].

Signaling Pathways >> Immunology/Inflammation >> Toll-like Receptor (TLR)

Research Areas >> Infection

Research Areas >> Inflammation/Immunology

ODN 2007 (1 or 5 μg/mL, 3-18 h) induces significantly chicken macrophage interferon IFN-γ and IFN-β expression[1]. ODN 2007 (10 μg/mL, 0.5-12 h) can increase levels of phosphorylation of ERK2 and AKT, stimulate the production of NO[3]. RT-PCR[1] Cell Line: MQ-NCSU cells (a chicken macrophage cell line) Concentration: 1 μg/mL, 5 μg/mL Incubation Time: 3 h, 12 h, 18 h Result: Significantly stimulated an increase in transcriptional expression of IFN-γ after 3 hours regardless of the dose. Increased the mRNA expression of IL-1β at 5 μg/mL high dose regardless of the time point. Western Blot Analysis[3] Cell Line: HD11, a replication-deficient avian leukemia virus MC29-transformed macrophage-like cell line Concentration: 10 μg/mL Incubation Time: 0.5 h, 1 h, 3 h, 6 h, 12 h Result: Resulted in a significant increase in ERK2 and AKT phosphorylation levels and increased IFN-γ, IL-6 and MIP-3α mRNA levels. Stimulated an increase in the level of NO.

ODN 2007 (intraperitoneal injection, 1 μg, once) can enhance the immune response by regulating the expression of immune system-related genes in zebrafish infected withVibrio traumaticus FJ03-X2[2]. Animal Model: Zebrafish infected with Vibrio traumaticus FJ03-X2[2] Dosage: 1 μg Administration: Intraperitoneal injection; once Result: Showed a cumulative mortality rate of 15.0% in treated group while the control group reached 42.1%. Showed a significant decrease in the expression levels of all intestinal immune-related genes such as TNF、IFNg1-2、IL-1β、IL-10, especially IL-1β decreased by 99.88%.

[1]. Tamiru N Alkie, et al. Characterization of Innate Responses Induced by PLGA Encapsulated- and Soluble TLR Ligands In Vitro and In Vivo in Chickens. PLoS One. 2017 Jan 3;12(1):e0169154.

[2]. Hua Chen, et al. CpG-ODN 2007 protects zebrafish (Danio rerio) against Vibrio vulnificus infection. Aquac Res. 2021; 52: 897-905.

[3]. Audesh Bhat, et al. Role of Hsp90 in CpG ODN mediated immunostimulation in avian macrophages. Mol Immunol. 2010 Mar;47(6):1337-46.