bis(8-hydroxyquinolinium) sulphate

Names

[ CAS No. ]:
495-99-8

[ Name ]:
bis(8-hydroxyquinolinium) sulphate

[Synonym ]:
2-Hydroxy-4,4'-stilbenedicarboxamidine
EINECS 207-811-0
Oxistilbamidinum
HYDROXYSTILBAMIDINE
2-Hydroxy-4,4'-diguanylstilbene

Biological Activity

[Description]:

Hydroxystilbamidine, a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases.

[In Vitro]

Hydroxystilbamidine permits the recovery of mRNA after polysome released with Nonidet P-40 (NP-40). Sucrose gradient analysis of detergent-lysed postnuclear supernates is used to analyze the size distribution of NP-40-released polysomes. The heparin gradient shows some polyribosomes, whereas the Hydroxystilbamidine gradient shows a remarkably large peak of very heavy polyribosomes. This peak is obtained reproducibly if Hydroxystilbamidine is present before the addition of NP-40[1].

[In Vivo]

Hydroxystilbamidine is an effective suppressor of the plaque-forming cell (PFC) response when given before sheep erythrocytes (SRBC) stimulation. Hydroxystilbamidine depresses the plaque response of the treated mice. Fewer PFC are observed in Hydroxystilbamidine-treated mice throughout the experiment, but the level of suppression decreases with time. By day 14, the number of PFC observed in both the Hydroxystilbamidine treated mice and the control group is essentially at the background level[2].

[References]

1. Lizardi PM. Isolation of giant silk fibroin polysomes and fibroin mRNP particles using a novel ribonuclease inhibitor, hydroxystilbamidine. J Cell Biol. 1980 Oct;87(1):292-6.

Chemical & Physical Properties

[ Density]:
1.288g/cm3

[ Boiling Point ]:
509.678ºC at 760 mmHg

[ Melting Point ]:
235°

[ Molecular Formula ]:
C₁₆H₁₆N₄O

[ Molecular Weight ]:
280.32400

[ Flash Point ]:
262.044ºC

[ Exact Mass ]:
280.13200

[ PSA ]:
118.98000

[ LogP ]:
1.57850

[ Vapour Pressure ]:
0mmHg at 25°C

[ Index of Refraction ]:
1.654

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: WJ5350000

CHEMICAL NAME :: 4,4'-Stilbenedicarboxamidine, 2-hydroxy-

CAS REGISTRY NUMBER :: 495-99-8

BEILSTEIN REFERENCE NO. :: 2700919

LAST UPDATED :: 199709

DATA ITEMS CITED :: 3

MOLECULAR FORMULA :: C16-H16-N4-O

MOLECULAR WEIGHT :: 280.36

WISWESSER LINE NOTATION :: MUYZR D1U1R DYZUM XQ

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Subcutaneous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 140 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 27 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

MUTATION DATA

TYPE OF TEST :: DNA adduct

TEST SYSTEM :: Mammal - species unspecified Lymphocyte

DOSE/DURATION :: 600 umol/L

REFERENCE :: BBACAQ Biochimica et Biophysica Acta. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1947- Volume(issue)/page/year: 407,43,1975