PIPES

Names

[ Name ]:
PIPES

[Synonym ]:
Sodium 2,2'-(1,4-piperazinediyl)diethanesulfonate 2-[4-(2-sulfoethyl)-1-piperazinyl]ethanesulfonate (3:1:1)
Piperazine-N,N'-bis (2-ethanesulfonic acid)
1,4-Piperazinediethanesulfonic acid, sodium salt (2:3)
PIPES
2,2'-Piperazine-1,4-diyldiethanesulfonic acid
2,2'-(1,4-Piperazinediyl)diethanesulfonic acid
1,4-Piperazine bis(ethanesulfonic acid)
1,4-Piperazinediethanesulfonic acid sesquisodium salt
Piperazine-N,N'-bis(2-ethanesulfonic acid)
Piperazine-N,N′-bis(2-ethanesulfonic acid)
Piperazine-1,4-bis(2-ethanesulfonic acid) sesquisodium salt
1,4-Piperazinediethanesulfonic acid
MFCD00006159
trisodium,2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonate,2-[4-(2-sulfonatoethyl)piperazin-1-yl]ethanesulfonate
EINECS 227-057-6
2,2′-piperazine-1,4-diylbisethanesulfonic acid

Biological Activity

[Description]:

PIPES (1,4-Piperazinediethanesulfonic acid) is an important component of PIPES buffer agent used in biochemistry[1].

[Related Catalog]:

Research Areas >> Cancer

Signaling Pathways >> Others >> Others

Research Areas >> Inflammation/Immunology

[In Vitro]

To prepare the pH PIPES buffer, 173 g of 1,4-piperazinediethanesulfonic acid are dissolved into 1 L of deionized water. The pH of the PIPES buffer is adjusted to 6.8 by adding pellets of sodium hydroxide. PIPES buffer can be used to PIPES buffer, it can prevent the glutaraldehyde fixation induced lipid loss and artifacts[1].

[References]

[1]. Jason Moggridge, et al. Sensitive Detection of Immunoglobulin G Stability Using in Real-Time Isothermal Differential Scanning Fluorimetry: Determinants of Protein Stability for Antibody-Based Therapeutics. Technol Cancer Res Treat. 2017 Dec;16(6):997-1005.

Chemical & Physical Properties

[ Density]:
1.5±0.1 g/cm3

[ Melting Point ]:
>300 °C(lit.)

[ Molecular Formula ]:
C₁₆H₃₃N₄Na₃O₁₂S₄

[ Molecular Weight ]:
670.682

[ Exact Mass ]:
670.067078

[ PSA ]:
131.98000

[ LogP ]:
-4.21

[ Index of Refraction ]:
1.566

[ Storage condition ]:
Store at RT.

[ Water Solubility ]:
0.1 M NaOH: 0.25 g/mL, clear, colorless

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xi

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
S22-S24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ HS Code ]:
29335995

Customs

[ HS Code ]: 2933599090

[ Summary ]:
2933599090. other compounds containing a pyrimidine ring (whether or not hydrogenated) or piperazine ring in the structure. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

Articles

Effect of (2)H and (18)O water isotopes in kinesin-1 gliding assay.

PeerJ 2 , e284, (2014)

We show for the first time the effects of heavy-hydrogen water ((2)H2O) and heavy-oxygen water (H2 (18)O) on the gliding speed of microtubules on kinesin-1 coated surfaces. Increased fractions of isot...

Evidence that TSC2 acts as a transcription factor and binds to and represses the promoter of Epiregulin.

Nucleic Acids Res. 42(10) , 6243-55, (2014)

The TSC2 gene, mutated in patients with tuberous sclerosis complex (TSC), encodes a 200 kDa protein TSC2 (tuberin). The importance of TSC2 in the regulation of cell growth and proliferation is irrefut...

Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination.

Cell. Mol. Life Sci. 71(24) , 4895-910, (2014)

Membrane proteins are key elements in cell physiology and drug targeting, but getting a high-resolution structure by crystallographic means is still enormously challenging. Novel strategies are in big...