EMA [Ethidium monoazide bromide]

Names

[ Name ]:
EMA [Ethidium monoazide bromide]

[Synonym ]:
Phenanthridinium,3-amino-8-azido-5-ethyl-6-phenyl-,bromide
MFCD00213986
3-Amino-8-azido-5-ethyl-6-phenylphenanthridinium bromide
Ethidium azide bromide
Ethidium bromide monoazide
Ethidium monoazide bromide
Monoazido ethidium bromide

Biological Activity

[Description]:

Ethidium monoazide bromide is a DNA intercalating fluorescent dye that enters bacteria with damaged membranes. Ethidium monoazide bromide can be covalently linked to DNA by photoactivation. Ethidium monoazide bromide stains only dead cells[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[References]

[1]. Nogva HK, et al. Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR. Biotechniques. 2003 Apr;34(4):804-8, 810, 812-3.

Chemical & Physical Properties

[ Molecular Formula ]:
C₂₁H₁₈BrN₅

[ Molecular Weight ]:
420.30500

[ Exact Mass ]:
419.07500

[ PSA ]:
79.65000

[ LogP ]:
2.52936

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: SF7941850

CHEMICAL NAME :: Phenanthridinium, 3-amino-8-azido-5-ethyl-6-phenyl-, bromide

CAS REGISTRY NUMBER :: 58880-05-0

LAST UPDATED :: 199707

DATA ITEMS CITED :: 2

MOLECULAR FORMULA :: C21-H18-N5.Br

MOLECULAR WEIGHT :: 420.35

WISWESSER LINE NOTATION :: T B666 HKJ EZ H2 IR& LNNN &E

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

MUTATION DATA

TYPE OF TEST :: Mutation in microorganisms

TEST SYSTEM :: Yeast - Saccharomyces cerevisiae

DOSE/DURATION :: 2 umol/L/30M

REFERENCE :: MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 56,21,1977

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ RIDADR ]:
NONH for all modes of transport

[ RTECS ]:
SF7941850

Articles

Modified vaccinia virus Ankara-infected dendritic cells present CD4+ T-cell epitopes by endogenous major histocompatibility complex class II presentation pathways.

J. Virol. 89(5) , 2698-709, (2015)

CD4(+) T lymphocytes play a central role in the immune system and mediate their function after recognition of their respective antigens presented on major histocompatibility complex II (MHCII) molecul...

Propidium monoazide (PMA) and ethidium bromide monoazide (EMA) improve DNA array and high-throughput sequencing of porcine reproductive and respiratory syndrome virus identification.

J. Virol. Methods 222 , 182-91, (2015)

Pan-viral DNA array (PVDA) and high-throughput sequencing (HTS) are useful tools to identify novel viruses of emerging diseases. However, both techniques have difficulties to identify viruses in clini...

Extracellular matrices decellularized from embryonic stem cells maintained their structure and signaling specificity.

Tissue Eng. Part A 20(1-2) , 54-66, (2014)

Embryonic stem cells (ESCs) emerge as a promising tool for tissue engineering and regenerative medicines due to their extensive self-renewal ability and the capacity to give rise to cells from all thr...