4-Methylumbelliferylglucuronide

Names

[ Name ]:
4-Methylumbelliferylglucuronide

[Synonym ]:
T66 BOVJ E1 IO- BT6OTJ CQ DQ EQ FVQ &&β-D-Gluco Form
(2S,3S,4S,5R,6S)-3,4,5-trihydroxy-6-(4-methyl-2-oxochromen-7-yl)oxyoxane-2-carboxylic acid
4-Methylumbelliferyl β-glucuronide
β-D-Glucopyranosiduronic acid, 4-methyl-2-oxo-2H-1-benzopyran-7-yl
2H-1-Benzopyran-2-one, 7-(β-D-glucopyranuronosyloxy)-4-methyl-
MFCD00036772
EINECS 228-186-0
4-Methylumbelliferyl-B-D-glucuronic acid
4-Methylumbelliferone β-D-glucuronide
4-Methylumbelliferylglucuronide
4-Methylumbelliferyl-β-D-glucuronide
4-Methyl-2-oxo-2H-chromen-7-yl β-D-glucopyranosiduronic acid
4'-Methylumbelliferyl-ß-D-glucuronide
4-methyl-2-oxo-2H-1-benzopyran-7-yl β-D-glucopyranosiduronic acid

Biological Activity

[Description]:

4-Methylumbelliferyl-β-D-glucuronide is a fluorogenic substrat (λex=362 nm, λem=445 nm). 4-Methylumbelliferyl-β-D-glucuronide has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli[1][2][3].

[Related Catalog]:

Research Areas >> Others

[In Vitro]

4-Methylumbelliferyl-β-D-glucuronide (156 µM-5 mM；30 min) 在 37°C 下释放 4-甲基伞形酮，用于酶促反应[2]。 4-Methylumbelliferyl-β-D-glucuronide (50 µg/mL；24 h) 在 TSA 培养基中用于检测大肠杆菌数[3]。

[References]

[1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5.

[2]. Sperker B, et al. High-performance liquid chromatographic quantification of 4-methylumbelliferyl-beta-D-glucuronide as a probe for human beta-glucuronidase activity in tissue homogenates. J Chromatogr B Biomed Appl. 1996 Oct 11;685(1):181-4.

[3]. Villari P, et al. An evaluation of the use of 4-methylumbelliferyl-beta-D-glucuronide (MUG) in different solid media for the detection and enumeration of Escherichia coli in foods. Lett Appl Microbiol. 1997 Apr;24(4):286-90.

Chemical & Physical Properties

[ Density]:
1.6±0.1 g/cm3

[ Boiling Point ]:
683.1±55.0 °C at 760 mmHg

[ Melting Point ]:
102 °C

[ Molecular Formula ]:
C₁₆H₁₆O₉

[ Molecular Weight ]:
352.293

[ Flash Point ]:
255.0±25.0 °C

[ Exact Mass ]:
352.079437

[ PSA ]:
146.66000

[ LogP ]:
-0.63

[ Vapour Pressure ]:
0.0±2.2 mmHg at 25°C

[ Index of Refraction ]:
1.664

[ Storage condition ]:
2~8°C

[ Water Solubility ]:
slightly soluble

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xi: Irritant;

[ Risk Phrases ]:
R22

[ Safety Phrases ]:
S24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Articles

Development of an enzyme assay for rapid assessment of Escherichia coli in seawaters.

J. Appl. Microbiol. 93(4) , 548-56, (2002)

An analytical protocol has been developed and applied for the detection of glucuronidase activity in marine waters as a rapid alternative approach to assess the microbiological quality of seawaters.Th...

An alternative approach for enumeration of Escherichia coli in foods.

Int. J. Food Microbiol. 68(3) , 217-23, (2001)

An assay to screen Escherichia coli in foods using MUG supplemented lauryl sulfate tryptose (LST) broth instead of tryptone water (TW) medium was evaluated. The results presented in this paper suggest...

Serogroup distribution and virulence characteristics of sorbitol-negative Escherichia coli from food and cattle stool.

J. Appl. Microbiol. 108(2) , 658-65, (2010)

To (i) study the serogroup distribution and virulence characteristics of non-sorbitol-fermenting Escherichia coli isolates from foods of animal origin and cattle faeces and (ii) re-examine the true so...