L-LEUCINE-7-AMINO-4-METHYLCOUMARIN HCL

Names

[ Name ]:
L-LEUCINE-7-AMINO-4-METHYLCOUMARIN HCL

[Synonym ]:
MFCD00043262
2-amino-4-methyl-N-(4-methyl-2-oxochromen-7-yl)pentanamide,hydrochloride
H-Leu-AMC.HCL

Biological Activity

[Description]:

L-Leucine-7-amido-4-methylcoumarin (Leu-AMC) hydrochloride is a bright blue fluorogenic peptidyl substrate for LAP3 (leucine aminopeptidase). L-Leucine-7-amido-4-methylcoumarin hydrochloride can be used for leucine aminopeptidase inhibition assays in vitro[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

In leucine aminopeptidases inhibition assays in KB 3.1 cells. Compounds 3 and 5 are observed to weakly inhibit the biofilm formation by S. aureus and constrain inhibitory activity of ʟ-Leu-AMC hydrolysis in KB 3.1 cells[1]. The amount of hydrolyzed 7-amino-4-methylcoumarin (AMC) is determined in a fluorescence spectrophotometer (excitation/emission: 365/440 nm)[1].

[References]

[1]. Tian Cheng, et al. Skeletocutins M-Q: biologically active compounds from the fruiting bodies of the basidiomycete Skeletocutis sp. collected in Africa. Beilstein J Org Chem

Chemical & Physical Properties

[ Boiling Point ]:
503ºC at 760 mmHg

[ Melting Point ]:
248ºC

[ Molecular Formula ]:
C₁₆H₂₁ClN₂O₃

[ Molecular Weight ]:
324.80300

[ Flash Point ]:
258ºC

[ Exact Mass ]:
324.12400

[ PSA ]:
85.33000

[ LogP ]:
3.98860

[ Storage condition ]:
-20°C

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Safety Phrases ]:
S22-S24/25

[ RIDADR ]:
NONH for all modes of transport

[ HS Code ]:
2932209090

Precursor & DownStream

Precursor

DownStream

Customs

[ HS Code ]: 2932209090

[ Summary ]:
2932209090. other lactones. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

Articles

Screening the Medicines for Malaria Venture "Malaria Box" against the Plasmodium falciparum aminopeptidases, M1, M17 and M18.

PLoS ONE 10(2) , e0115859, (2015)

Malaria is a parasitic disease that remains a global health burden. The ability of the parasite to rapidly develop resistance to therapeutics drives an urgent need for the delivery of new drugs. The M...

Enzyme-based resource allocated decomposition and landscape heterogeneity in the Florida Everglades.

J. Environ. Qual. 37 , 972-6, (2008)

Enzyme catalyzed reactions are generally considered the rate-limiting step in organic matter degradation and may be significantly influenced by the structure and composition of plant communities. Chan...