LONGISTYLINE A

Longistyline A (Longistylin A) is a natural stilbene, it can be isolated from leaves of Cajanus cajan. Longistyline A shows antimicrobial activity against MRSA with an MIC value of 1.56 μg/mL. Longistyline A shows neuroprotective effects, it can be used for the research of infection and nerve diseases[1][2].

Research Areas >> Infection

Research Areas >> Neurological Disease

Signaling Pathways >> Anti-infection >> Bacterial

IC50: 8.61 μg/mL (murine RAW264.7 cells)[2]

Longistyline A (4-16 μM/L; 48 h) reduces the corticosterone-induced neurotoxicity in PC12 cells[1]. Longistyline A can function as a membrane-targeting antibacterial agent by disturbing bacterial membrane potential and increasing the permeation[2]. Longistyline A (0-100 μg/mL) shows significant antimicrobial activity against tested Gram-positive bacteria, such as B.cereus CMCC63302 with MIC values of 25 μg/mL, S.aureus CMCC26003 with MIC values of 1.56 μg/mL, and both two strains of MRSA (JCSC4744 and JCSC4469) with MIC values of 1.56 μg/mL[2]. Longistyline A (24 h) shows cytotoxicity towards murine RAW264.7 cells with an IC50 value of 8.61 μg/mL[2]. Cell Cytotoxicity Assay[1] Cell Line: PC12 cell line Concentration: 4, 8 and 16 μM/L Incubation Time: 48 hours Result: Generated a neuroprotective effect against corticosterone-induced neurotoxicity in PC12 cells possibly by decreasing [Ca2+]i and caspase-3 activity.

Longistyline A (10 µg dissolved in 50 µL PBS buffer; apply to the wound; for 30 min) improves wound healing, decreases MRSA bacterial survivals in the lesions and reduces inflammatory reaction in MRSA infected mice[2]. Animal Model: Female wound healing BALB/c mice with MRSA infection[2] Dosage: 10 µg Administration: Apply to the wound; 10 µg dissolved in 50 µL PBS buffer; 5 min after infection for 30 min Result: Showed a wound healing efficiency and significantly alleviated weight loss induced by MRSA infection. Reduced gram-positive microorganisms in vivo with a better effect than vancomycin. Reduced accumulation of immune cells at the injury site and alleviated induction of TNF-α and IL-6 in serum.