Boc-Leu-Gly-Arg-AMC acetate salt

Boc-Leu-Gly-Arg-AMC is a fluorogenic AMC substrate for the convertases. Boc-Leu-Gly-Arg-AMC can be used in enzymatic assays[1][2].

Research Areas >> Others

Signaling Pathways >> Others >> Others

To demonstrate the presence in the abdominal gland of proteolytic enzymes capable of generating Sodefrin, an enzymatic assay was developed using Boc-Leu-Gly-Arg-AMC as synthetic substrate. A crude extract of the abdominal gland hydrolyzed Boc-Leu-Gly-Arg-AMC to liberate 7-amino-4- methylcoumarin, suggesting that enzymes that generate sodefrin from its precursor molecule are present in the gland. The activity in the extract for cleaving Boc-Leu-Gly-Arg-AMC is optimal at pH 9.0 and 45 ℃[1].

[1]. Nakada T, et al. Evidence for processing enzymes in the abdominal gland of the newt, Cynops pyrrhogaster, that generate sodefrin from its biosynthetic precursor. Zoolog Sci. 2007 May;24(5):521-4.

[2]. Yumiko Obayashi, et al. Proteolytic enzymes in coastal surface seawater: Significant activity of endopeptidases and exopeptidases Limnol. Oceanogr., 50(2), 2005, 722-726.