7-Diethylamino-3-[4-(iodoacetamido)phenyl]-4-methylcoumarin

Names

[ Name ]:
7-Diethylamino-3-[4-(iodoacetamido)phenyl]-4-methylcoumarin

[Synonym ]:
n-{4-[7-(diethylamino)-4-methyl-2-oxo-2h-chromen-3-yl]phenyl}-2-iodoacetamide
Demcpi
7-DIETHYLAMINO-3-[4-(IODOACETAMIDO)PHENYL]-4-METHYLCOUMARIN
DCIA

Biological Activity

[Description]:

DCIA is a fluorescent dye. DCIA contains a coumarin fluorophore and conjugates specifically with free cysteines in the protein[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[References]

[1]. Branigan E, et al. Quantification of free cysteines in membrane and soluble proteins using a fluorescent dye and thermal unfolding. Nat Protoc. 2013 Nov;8(11):2090-7.

Chemical & Physical Properties

[ Density]:
1.531g/cm3

[ Boiling Point ]:
658.2ºC at 760 mmHg

[ Molecular Formula ]:
C₂₂H₂₃IN₂O₃

[ Molecular Weight ]:
490.33400

[ Flash Point ]:
351.9ºC

[ Exact Mass ]:
490.07500

[ PSA ]:
62.55000

[ LogP ]:
5.06110

[ Index of Refraction ]:
1.67

MSDS

Click to get detail MSDS

Safety Information

[ Symbol ]:

GHS06

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H301-H413

[ Precautionary Statements ]:
P301 + P310

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Faceshields;Gloves

[ RIDADR ]:
UN 2811 6.1 / PGIII

[ HS Code ]:
2932209090

Customs

[ HS Code ]: 2932209090

[ Summary ]:
2932209090. other lactones. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

Articles

High-performance liquid chromatography with chemiluminescence detection of serum levels of pre-column derivatized fluoropyrimidine compounds.

J. Chromatogr. A. 530(1) , 57-64, (1990)

7-(Diethylamino)-3-[4-[iodoacetyl)amino)phenyl]-4-methylcoumarin (DCIA) and 4-(bromomethyl)-7-methoxycoumarin have been evaluated as fluoropyrimidine-derivatizing agents to be detected using peroxyoxa...

Fluorescence characterization of the environment encountered by nascent polyalanine and polyserine as they exit Escherichia coli ribosomes during translation.

Biochemistry 31(8) , 2368-75, (1992)

The fate of the amino termini of nascent polyalanine, polyserine, and polylysine was monitored by fluorescence techniques as each was translated on Escherichia coli ribosomes. A coumarin probe was pla...

New fluorochromes for thiols: maleimide and iodoacetamide derivatives of a 3-phenylcoumarin fluorophore.

J. Histochem. Cytochem. 29(2) , 314-6, (1981)

N-(4-(7 - Diethylamino - methylcoumarin - 3 yl)phenyl)maleimide (CPM) and the corresponding iodoacetamide are described. When applied to sections at pH 6 and 9, respectively, the two fluorogens are ve...