BCECF

Names

[ CAS No. ]:
85138-49-4

[ Name ]:
BCECF

[Synonym ]:
2-[2,7-bis(2-carboxyethyl)-3-hydroxy-6-oxoxanthen-9-yl]terephthalic acid
BCECF

Biological Activity

[Description]:

BCECF is a duorescent dye with λexcitation of 420~520 nm and λemission of 530 nm.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

[In Vitro]

Fully treated cells show hydrogenosomes with an electron-dense deposit which aggregates to a variable extent.The staining is seen in the interior of hydrogenosomes in some instances. It is also observed by microscopy that the K+/H+ ionophor nigericin does not inhibit hydrogenosomal loading with BCECF[1].

[Kinase Assay]

The cells are palced in a petri dish and BCECF present in trichomonads is fixed for electron microsopy. Controns without BCECF loading, without DAB treatment, and without UV illumination are run in parallel. Trichomonads remaine viable during the procedure, except that without BCECF they are dead and disrupted by 4 h. Then the cell are lysed. After lysis, BCECF is added to the lysate to 20 μM, and the lysate is incubated,followed by 3 washes in lysis buffer to remove free dye and resuspension of the pellet in the original volume[1].

[References]

[1]. Scott DA, et al. Analysis of the uptake of the fluorescent marker 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF) by hydrogenosomes in Trichomonas vaginalis. Eur J Cell Biol. 1998 Jun;76(2):139-45.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.67g/cm3

[ Boiling Point ]:
936.2ºC at 760mmHg

[ Molecular Formula ]:
C₂₇H₂₀O₁₁

[ Molecular Weight ]:
520.44100

[ Flash Point ]:
318.2ºC

[ Exact Mass ]:
520.10100

[ PSA ]:
187.89000

[ LogP ]:
3.39840

[ Index of Refraction ]:
1.743

[ Storage condition ]:
-20°C

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xn

[ Risk Phrases ]:
20/21/22-36/37/38

[ Safety Phrases ]:
22-24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3.0

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