Cholesterol oxidase

Names

[ Name ]:
Cholesterol oxidase

[Synonym ]:
3-Hydroxysteroid oxidase
Cholesterol oxidase
MFCD00130783
Cholesterin oxidase

Biological Activity

[Description]:

Cholesterol oxidase (ChOx) is a bacterial flavin oxidase containing FAD, commonly used in biochemical research. Cholesterol oxidase catalyzes the oxidation of the C(3)-OH group of cholesterol (and other sterols) to cholest-5-en-3-one and isomerizes it to cholest-4-en-3-one[1].

[Related Catalog]:

Research Areas >> Others

[References]

[1]. Loredano Pollegioni,et al. Cholesterol oxidase: biotechnological applications. FEBS J. 2009 Dec;276(23):6857-70.

Chemical & Physical Properties

[ Appearance of Characters ]:
solution (slightly hazy) | pale yellow

[ Storage condition ]:
2-8°C

[ Water Solubility ]:
50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xi

[ Safety Phrases ]:
24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ HS Code ]:
35079090

Articles

Phospholipid-sepiolite biomimetic interfaces for the immobilization of enzymes.

ACS Appl. Mater. Interfaces 3(11) , 4339-48, (2011)

Biomimetic interfaces based on phosphatidylcholine (PC) assembled to the natural silicate sepiolite were prepared for the stable immobilization of the urease and cholesterol oxidase enzymes. This is a...

Electro-chemiluminescent biosensing.

Anal. Bioanal. Chem 390 , 155-68, (2008)

The present review draws a general picture of the bioanalytical applications of electro-chemiluminescent reactions (ECL). Only the two main ECL reactions-i.e. the luminol-based and Ru(bpy)(3)(2+)-base...

Effect of cholesterol concentration on organization of viral and vesicle membranes. Probed by accessibility to cholesterol oxidase.

J. Biol. Chem. 255(12) , 5802-6, (1980)