Broussochalcone A

Broussochalcone A is an antioxidant and an inhibitor of Xanthine Oxidase (IC50=2.21 μM), with free radical scavenging activity. Broussochalcone A inhibits iron-induced lipid peroxidation and nitric oxide synthesis in lipopolysaccharide (LPS) -activated macrophages. Broussochalcone A also induces Apoptosis of human renal carcinoma cells by increasing ROS levels and activating FOXO3 signaling pathways[1][2].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Research Areas >> Inflammation/Immunology

Signaling Pathways >> Metabolic Enzyme/Protease >> Xanthine Oxidase

Broussochalcone A (0.3, 1, and 3 μM; 10 min, 再 Fe 诱导 30 min) 在大鼠脑匀浆中抑制 Fe2+ (200 μM) 诱导的脂质过氧化[1]。 Broussochalcone A (1-30 μM; 30 min) 剂量依赖性增加 DPPH (100 μM) 的清除活性[1]。 Broussochalcone A (0.1-1 μM) 抑制细胞色素 c 还原，IC50 为 0.5 μM，这主要是由于其清除超氧阴离子活性，部分是由于其抑制黄嘌呤氧化酶活性[1]。 Broussochalcone A (1-20 μM; 24 h) 抑制亚硝酸盐的产生和 iNOS 蛋白的表达[1]。 Western Blot Analysis[1] Cell Line: LPS-activated RAW 264.7 macrophages Concentration: 1 μM, 3 μM, 10 μM, 20 μM Incubation Time: 30 min; then stimulated by LPS (1 μg/mL) for another 24 hr Result: Caused inhibition of iNOS protein expression dose-dependently. Inhibited IkBα phosphorylation.

[1]. Cheng Z, et al. Broussochalcone A, a potent antioxidant and effective suppressor of inducible nitric oxide synthase in lipopolysaccharide-activated macrophages. Biochem Pharmacol. 2001 Apr 15;61(8):939-46.  

[2]. Lee HK, et al. Broussochalcone A Induces Apoptosis in Human Renal Cancer Cells via ROS Level Elevation and Activation of FOXO3 Signaling Pathway. Oxid Med Cell Longev. 2021 Oct 27;2021:2800706.