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A Green-Light-Responsive System for the Control of Transgene Expression in Mammalian and Plant Cells
10.1021/acssynbio.7b00450 2018-04-19 The ever-increasing complexity of synthetic gene networks and applications of synthetic biology requires precise and orthogonal gene expression systems. Of particular interest are systems responsive to light as they enable the control of gene expression dynam... |
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Integrating T7 RNA Polymerase and Its Cognate Transcriptional Units for a Host-Independent and Stable Expression System in Single Plasmid
10.1021/acssynbio.8b00055 2018-04-19 Metabolic engineering and synthetic biology usually require universal expression systems for stable and efficient gene expression in various organisms. In this study, a host-independent and stable T7 expression system had been developed by integrating T7 RNA ... |
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Quantitative Characterization of Translational Riboregulators Using an in Vitro Transcription–Translation System
10.1021/acssynbio.7b00387 2018-04-18 Riboregulators are short RNA sequences that, upon binding to a ligand, change their secondary structure and influence the expression rate of a downstream gene. They constitute an attractive alternative to transcription factors for building synthetic gene regu... |
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Artificial Mating-Type Conversion and Repetitive Mating for Polyploid Generation
10.1021/acssynbio.8b00020 2018-04-17 The yeast Saccharomyces cerevisiae is one of the best-understood biological systems and can produce numerous useful compounds. Sexual hybridization (mating) can drive dramatic evolution of yeasts by the inheritance of half of the parental genomic information ... |
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A Transcription Factor-Based Biosensor for Detection of Itaconic Acid
10.1021/acssynbio.8b00057 2018-04-17 Itaconic acid is an important platform chemical that can easily be incorporated into polymers and has the potential to replace petrochemical-based acrylic or methacrylic acid. A number of microorganisms have been developed for the biosynthesis of itaconate in... |
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High GC Content Cas9-Mediated Genome-Editing and Biosynthetic Gene Cluster Activation in Saccharopolyspora erythraea
10.1021/acssynbio.7b00448 2018-04-17 The overexpression of bacterial secondary metabolite biosynthetic enzymes is the basis for industrial overproducing strains. Genome editing tools can be used to further improve gene expression and yield. Saccharopolyspora erythraea produces erythromycin, whic... |
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Rerouting of Metabolism into Desired Cellular Products by Nutrient Stress: Fluxes Reveal the Selected Pathways in Cyanobacterial Photosynthesis
10.1021/acssynbio.8b00116 2018-04-17 Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways an... |
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Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids
10.1021/acssynbio.7b00399 2018-04-13 Engineering the bacteria present in animal microbiomes promises to lead to breakthroughs in medicine and agriculture, but progress is hampered by a dearth of tools for genetically modifying the diverse species that comprise these communities. Here we present ... |
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Engineered Reciprocal Chromosome Translocations Drive High Threshold, Reversible Population Replacement in Drosophila
10.1021/acssynbio.7b00451 2018-04-13 Replacement of wild insect populations with transgene-bearing individuals unable to transmit disease or survive under specific environmental conditions using gene drive provides a self-perpetuating method of disease prevention. Mechanisms that require the gen... |
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MIDAS: A Modular DNA Assembly System for Synthetic Biology
10.1021/acssynbio.7b00363 2018-04-12 A modular and hierarchical DNA assembly platform for synthetic biology based on Golden Gate (Type IIS restriction enzyme) cloning is described. This enabling technology, termed MIDAS (for Modular Idempotent DNA Assembly System), can be used to precisely assem... |