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赤藓红B

赤藓红B用途

用于食品、药物和化妆品的着色

赤藓红B名称

[ CAS 号 ]:
16423-68-0

[ 中文名 ]:
赤藓红B

[ 英文名 ]:
erythrosin B

[中文别名 ]:

[英文别名 ]:

1427red
Erythrosin B,high purity,biological stain
Erythrosin
Tetraiodofluorescein sodium salt
Erythrosine Sodium (close form)
2',4',5',7'-Tetraiodofluorescein disodium salt
Erythrosin extra bluish
IODOEOSIN
FD & C Red 3
EINECS 240-474-8

赤藓红B生物活性

[ 描述 ]:

Erythrosin B 是广泛用于食品和纺织工业的人造染料。Erythrosin B 也是一种新型的光敏剂,已被用于开发动物模型。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[体外研究]

当分析代表DNA损伤程度的尾部力矩和尾部强度时,仅测试的两种最高浓度的赤藓红B(50.0和70.0μg/ mL)与载体对照组显着不同(p <0.05)。结果显示,与载体对照组相比,7种Erythrosin B浓度中的6种(0.2至70.0μg/ mL)的微核(MNi)频率增加[1]。

[细胞实验]

进行碱性单细胞凝胶电泳测定（彗星测定）。简而言之，将2×105个HepG2细胞接种在24孔板中24小时。然后用Erythrosin B以0.1,0.2,2.0,10.0,25.0,50.0或70.0μg/ mL（终浓度）处理细胞4小时;还进行载体对照（0.7％DMSO）和阳性对照（多柔比星0.3μg/ mL）处理。将HepG2细胞悬浮液与37℃低熔点琼脂糖混合，并转移至正常熔点琼脂糖涂覆的载玻片上。每次处理分析100个随机选择的类核，并进行总共三次独立实验[1]。

[参考文献]

[1]. Chen W, et al. Establishing an experimental rat model of photodynamically-induced retinal vein occlusion using erythrosin B. Int J Ophthalmol. 2014 Apr 18;7(2):232-8.

[2]. Chequer FM, et al. Genotoxic and mutagenic effects of erythrosine B, a xanthene food dye, on HepG2 cells. Food Chem Toxicol. 2012 Oct;50(10):3447-51.

[相关活性小分子]

赤藓红B物理化学性质

[ 密度 ]:
0.98

[ 沸点 ]:
693.2ºC at 760 mmHg

[ 熔点 ]:
>300ºC (Decomposes)

[ 分子式 ]:
C₂₀H₆I₄Na₂O₅

[ 分子量 ]:
879.856

[ 闪点 ]:
373ºC

[ 精确质量 ]:
879.618896

[ PSA ]:
81.65000

[ LogP ]:
6.96060

[ 外观性状 ]:
栗色粉末

[ 蒸汽压 ]:
2.22E-16mmHg at 25°C

[ 储存条件 ]:
Store at +5°C to +30°C.

[ 水溶解性 ]:
100g/l

赤藓红BMSDS

详细MSDS(安全技术说明书)

赤藓红B毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: LM5950000

CHEMICAL NAME :: Fluorescein, 2',4',5',7'-tetraiodo-, disodium salt

CAS REGISTRY NUMBER :: 16423-68-0

LAST UPDATED :: 199710

DATA ITEMS CITED :: 31

MOLECULAR FORMULA :: C20-H6-I4-O5.2Na

MOLECULAR WEIGHT :: 879.84

WISWESSER LINE NOTATION :: T C666 BO EVJ DI FI IR BVQ& LI MQ NI &-NA- 2

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 1840 mg/kg

TOXIC EFFECTS :: Behavioral - convulsions or effect on seizure threshold

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 300 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 200 mg/kg

TOXIC EFFECTS :: Behavioral - convulsions or effect on seizure threshold

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Unreported

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 1895 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 1264 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 370 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 200 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - gerbil

DOSE/DURATION :: 1930 mg/kg

TOXIC EFFECTS :: Behavioral - convulsions or effect on seizure threshold

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 90 gm/kg/90D-C

TOXIC EFFECTS :: Gastrointestinal - other changes Endocrine - changes in thyroid weight

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Mammal - pig

DOSE/DURATION :: 147 gm/kg/14W-I

TOXIC EFFECTS :: Endocrine - changes in thyroid weight Blood - changes in leukocyte (WBC) count

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 1798 gm/kg/2Y-C

TOXIC EFFECTS :: Tumorigenic - neoplastic by RTECS criteria Endocrine - thyroid tumors

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 482 gm/kg

SEX/DURATION :: multigeneration

TOXIC EFFECTS :: Reproductive - Effects on Newborn - growth statistics (e.g.%, reduced weight gain)

MUTATION DATA

TYPE OF TEST :: Cytogenetic analysis

TEST SYSTEM :: Rodent - hamster Lung

DOSE/DURATION :: 1100 mg/L

REFERENCE :: GMCRDC Gann Monograph on Cancer Research. (Plenum Pub. Corp., 233 Spring St., New York, NY 10013) No. 11- 1971- Volume(issue)/page/year: 27,95,1981 *** U.S. STANDARDS AND REGULATIONS *** EPA FIFRA 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTRATION FEREAC Federal Register. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402) V.1- 1936- Volume(issue)/page/year: 54,7740,1989 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M4011 No. of Facilities: 904 (estimated) No. of Industries: 5 No. of Occupations: 11 No. of Employees: 8221 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M4011 No. of Facilities: 2991 (estimated) No. of Industries: 17 No. of Occupations: 39 No. of Employees: 67011 (estimated) No. of Female Employees: 25006 (estimated)

赤藓红B安全信息

[ 符号 ]:

GHS07

[ 信号词 ]:
Warning

[ 危害声明 ]:
H302-H413

[ 个人防护装备 ]:
dust mask type N95 (US);Eyeshields;Gloves

[ 危害码 (欧洲) ]:
Xn:Harmful

[ 风险声明 (欧洲) ]:
R22

[ 安全声明 (欧洲) ]:
36

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

[ RTECS号 ]:
LM5950000

[ 海关编码 ]:
32129000

赤藓红B制备

【方法1】
由间苯二酚、邻苯二甲酸酐及无水氯化锌加热熔融而得的粗制荧光素，经精制、碘化后，用氯化钠盐析，再经精制而得。
【方法2】
由苯甲醛-4-磺酸与二乙基间氨基苯酚缩合生成的产物，经浓硫酸脱水，用重铬酸钾、高锰酸盐或氯化铁氧化，制成钠盐。
【方法3】
赤藓红的制备
将间苯二酚加热熔融，加入邻苯二甲酸酐搅匀熔化，加热至185℃反应半小时，生成深黄色的间苯二酚酞，此时呈液体状态。在搅拌下加入粉末状无水氯化锌，搅拌至完全溶解，并逐步升温至210～215℃，加热至反应物完全固化，再冷却、粉碎得粗融物；将此粗融物与稀盐酸加热共沸，浸出氯化锌和残留的间苯二酚，过滤、水洗、干燥后，再用乙醇提取未反应的间苯二酚酞，提取过的产物经干燥即得荧光素。
将荧光素溶解于氢氧化钠溶液中，加碘进行反应，反应物中加入盐酸，析出结晶，然后将其转化为钠盐溶液，浓缩即得产品。赤藓红铝色淀的制备
由氯化铝、硫酸铝等的铝盐与碳酸钠等的碱类制取氢氧化铝，然后加于赤藓红水溶液中，沉淀而得产品。

赤藓红B文献

Gold(I) complexes of 9-deazahypoxanthine as selective antitumor and anti-inflammatory agents.

PLoS ONE 9(10) , e109901, (2014)

The gold(I) mixed-ligand complexes involving O-substituted derivatives of 9-deazahypoxanthine (HLn) and triphenylphosphine (PPh3) with the general formula [Au(Ln)(PPh3)] (1-5) were prepared and thorou...

Evaluation of potential genotoxicity of five food dyes using the somatic mutation and recombination test.

Chemosphere 88(8) , 974-9, (2012)

In this study, different concentrations of five food dyes (amaranth, patent blue, carminic acid, indigotine and erythrosine) have been evaluated for genotoxicity in the Somatic Mutation and Recombinat...

Hypoxia enhances proliferation and stemness of human adipose-derived mesenchymal stem cells.

Cytotechnology 67 , 1073-84, (2015)

The aim of the study was to obtain the highest number of multipotent adipose-derived mesenchymal stem cells (ADMSCs) by using culture conditions which favour cell expansion without loss of mesenchymal...

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