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嘌呤霉素二盐酸盐

嘌呤霉素二盐酸盐用途

Puromycin dihydrochloride是嘌呤霉素的二盐酸盐。 Puromycin是一种抑制 protein synthesis 的氨基糖苷类抗生素。

嘌呤霉素二盐酸盐名称

[ CAS 号 ]:
58-58-2

[ 中文名 ]:
嘌呤霉素盐酸盐

[ 英文名 ]:
Puromycin 2HCl

[中文别名 ]:

[英文别名 ]:

PUROMYCIN
EINECS 200-387-8
MFCD00067313
PUROMYCIN HYDROCHLORIDE
Puromycin dihydrochloride
puromycinum [INN_la]
P 638 dihydrochloride
3123-L
Stylomycin dihydrochloride
Stillomycin

嘌呤霉素二盐酸盐生物活性

[ 描述 ]:

Puromycin dihydrochloride是嘌呤霉素的二盐酸盐。 Puromycin是一种抑制 protein synthesis 的氨基糖苷类抗生素。

[ 相关类别 ]:

信号通路 >> 抗感染 >> 细菌

天然产物 >> 其他

研究领域 >> 感染

[体外研究]

嘌呤霉素在氨酰基-sRNA形成后阻断蛋白质合成,同时它导致小肽的积累。这两种效应似乎都是由于核糖体结合的肽基-sRNA的分裂,导致不完整肽链的释放[1]。嘌呤霉素是氨酰基-tRNA的3'末端的类似物,通过与生长的多肽链非特异性连接而导致翻译过早终止。嘌呤霉素具有两种抑制作用模式。第一种是通过作为受体底物,其攻击P位点中的肽基-tRNA以形成新生肽。第二种是通过与氨酰基-tRNA竞争结合A'位点[2]。当以最小量使用时,嘌呤霉素掺入新合成的蛋白质直接反映体外mRNA翻译的速率。嘌呤霉素免疫检测是放射性氨基酸标记的有利替代方案。它允许通过免疫荧光显微镜直接评估单细胞中的翻译活性,并通过荧光激活细胞分选直接评估异质细胞群[3]。

[参考文献]

[1]. Nathans D, et al. Puromycin inhibition of protein synthesis: incorporation of puromycin intopeptide chains. Proc Natl Acad Sci U S A. 1964 Apr;51:585-92.

[2]. Miyamoto-Sato E, et al. Specific bonding of puromycin to full-length protein at the C-terminus. Nucleic Acids Res. 2000 Mar 1;28(5):1176-82.

[3]. Schmidt EK, et al. SUnSET, a nonradioactive method to monitor protein synthesis. Nat Methods. 2009 Apr;6(4):275-7.

[相关活性小分子]

嘌呤霉素二盐酸盐物理化学性质

[ 密度 ]:
1.5±0.1 g/cm3

[ 熔点 ]:
168-170℃

[ 分子式 ]:
C₂₂H₃₁Cl₂N₇O₅

[ 分子量 ]:
544.43

[ PSA ]:
160.88000

[ LogP ]:
0.93

[ 外观性状 ]:
白色粉末

[ 折射率 ]:
1.701

[ 储存条件 ]:
−20°C

[ 稳定性 ]:
Stable. Heat sensitive. Incompatible with strong oxidizing agents.

[ 水溶解性 ]:
H2O: soluble50mg/mL (Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at 20 °C.) | Soluble in water

嘌呤霉素二盐酸盐MSDS

详细MSDS(安全技术说明书)

嘌呤霉素二盐酸盐毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: AU7355000

CHEMICAL NAME :: Adenosine, 3'-((2-amino-3-(4-methoxyphenyl)-1-oxopropyl)amino)-3 '-deoxy-N,N-dimethyl-, Dihydrochloride, (S)-

CAS REGISTRY NUMBER :: 58-58-2

LAST UPDATED :: 199801

DATA ITEMS CITED :: 11

MOLECULAR FORMULA :: C22-H29-N7-O5.2Cl-H

MOLECULAR WEIGHT :: 544.50

WISWESSER LINE NOTATION :: T56 BN DN FN HNJ IN1&1 D- BT5OTJ CQ DMVYZ1R DO1& E1Q &GH

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 720 mg/kg

TOXIC EFFECTS :: Nutritional and Gross Metabolic - weight loss or decreased weight gain Lungs, Thorax, or Respiration - respiratory depression Gastrointestinal - hypermotility, diarrhea

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 580 mg/kg

TOXIC EFFECTS :: Nutritional and Gross Metabolic - weight loss or decreased weight gain Lungs, Thorax, or Respiration - respiratory depression Gastrointestinal - hypermotility, diarrhea

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 335 mg/kg

TOXIC EFFECTS :: Nutritional and Gross Metabolic - weight loss or decreased weight gain Lungs, Thorax, or Respiration - respiratory depression Gastrointestinal - hypermotility, diarrhea

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - guinea pig

DOSE/DURATION :: 600 mg/kg

TOXIC EFFECTS :: Nutritional and Gross Metabolic - weight loss or decreased weight gain Behavioral - muscle weakness

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - guinea pig

DOSE/DURATION :: 287 mg/kg

TOXIC EFFECTS :: Nutritional and Gross Metabolic - weight loss or decreased weight gain Behavioral - muscle weakness

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intramuscular

SPECIES OBSERVED :: Rodent - guinea pig

DOSE/DURATION :: 202 mg/kg

TOXIC EFFECTS :: Nutritional and Gross Metabolic - weight loss or decreased weight gain Behavioral - muscle weakness

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 1 gm/kg/10D-I

TOXIC EFFECTS :: Behavioral - somnolence (general depressed activity) Gastrointestinal - hypermotility, diarrhea Nutritional and Gross Metabolic - weight loss or decreased weight gain

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intramuscular

SPECIES OBSERVED :: Mammal - dog

DOSE/DURATION :: 150 mg/kg/3D-I

TOXIC EFFECTS :: Gastrointestinal - nausea or vomiting Related to Chronic Data - death

TYPE OF TEST :: DNA inhibition

TYPE OF TEST :: Mutation test systems - not otherwise specified

MUTATION DATA

TEST SYSTEM :: Rodent - mouse

DOSE/DURATION :: 100 mg/kg

REFERENCE :: FOBLAN Folia Biologica (Prague). (Academic Press Inc. Ltd., 24-28 Oval Rd., London NW1 7DX, UK) V.1- 1955- Volume(issue)/page/year: 21,60,1975

嘌呤霉素二盐酸盐安全信息

[ 符号 ]:

GHS07

[ 信号词 ]:
Warning

[ 危害声明 ]:
H302

[ 个人防护装备 ]:
Eyeshields;Faceshields;Gloves;half-mask respirator (US);multi-purpose combination respirator cartridge (US)

[ 危害码 (欧洲) ]:
Xn

[ 风险声明 (欧洲) ]:
R22

[ 安全声明 (欧洲) ]:
S36

[ 危险品运输编码 ]:
UN 3249

[ WGK德国 ]:
3

[ RTECS号 ]:
AU7355000

[ 包装等级 ]:
III

[ 危险类别 ]:
6.1(b)

嘌呤霉素二盐酸盐制备

由白色链球菌（Streptomyces.alboniger）发酵制得。

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