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An Unusual Fatty Acyl:Adenylate Ligase (FAAL)–Acyl Carrier Protein (ACP) Didomain in Ambruticin Biosynthesis
10.1002/cbic.201800084 2018-04-18 The divinylcyclopropane (DVC) fragment of the ambruticins is proposed to be formed by a unique polyene cyclisation mechanism, in which the unusual didomain AmbG plays a key role. It is proposed to activate the branched thioester carboxylic acid resulting from... |
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Amide Neighbouring‐Group Effects in Peptides: Phenylalanine as Relay Amino Acid in Long‐Distance Electron Transfer
10.1002/cbic.201800098 2018-04-14 In nature, proteins serve as media for long‐distance electron transfer (ET) to carry out redox reactions in distant compartments. This ET occurs either by a single‐step superexchange or through a multi‐step charge hopping process, which uses side chains of am... |
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Light‐Dependent Cytoplasmic Recruitment Enhances the Dynamic Range of a Nuclear Import Photoswitch
10.1002/cbic.201700681 2018-04-06 Cellular signal transduction is often regulated at multiple steps to achieve more complex logic or precise control of a pathway. For instance, some signaling mechanisms couple allosteric activation with localization to achieve high signal to noise. Here, we c... |
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Dual Labeling of the CBP/p300 KIX Domain for 19F NMR Leads to Identification of a New Small‐Molecule Binding Site
10.1002/cbic.201700686 2018-04-06 Protein‐Observed Fluorine NMR (PrOF NMR) spectroscopy is an emerging technique for screening and characterizing small‐molecule–protein interactions. The choice of which amino acid to label for PrOF NMR can be critical for analysis. Here we report the first us... |
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Peptide‐based scaffold for nitric oxide induced differentiation of neuroblastoma cells
10.1002/cbic.201800065 2018-03-30 Nitric oxide (NO) is a gaseous messenger involved in neuronal differentiation, development and synaptogenesis, in addition to many other physiological functions. Therefore, it is imperative to maintain optimal nitric oxide concentration to ensure its biochemi... |
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Characterization of the actinonin biosynthetic gene cluster
10.1002/cbic.201800116 2018-03-30 The hydroxamate moiety of the natural product actinonin mediates inhibition of metalloproteinases because of its chelating properties towards divalent cations in the active site of those enzymes. Due to its antimicrobial activity actinonin has served as a lea... |
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Reaction mechanism of prephenate dehydrogenase from the alternate tyrosine biosynthesis pathway in plants
10.1002/cbic.201800085 2018-03-30 Unlike metazoans, plants, bacteria, and fungi retain the enzymatic machinery necessary to synthesize the three aromatic amino acids, L¬‐phenylalanine, L‐tyrosine, and L‐tryptophan de novo. In legumes, such as soybean, alfalfa, and common bean, prephenate dehy... |
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Protein crystallography and site‐direct mutagenesis analysis of the poly(ethylene terephthalate) hydrolase PETase from Ideonella sakaiensis
10.1002/cbic.201800097 2018-03-30 Compared with traditional recycle strategies, biodegradation provides a sustainable solution for poly (ethylene terephthalate) (PET) wastes disposal. PETase, a newly identified enzyme from Ideonella sakaiensis, has high efficiency and specificity towards PET,... |
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Directed Evolution of Hyaluronic Acid Synthase from Pasteurella multocida Towards High Molecular Weight Hyaluronic Acid
10.1002/cbic.201800093 2018-03-30 Hyaluronic acid (HA) is the natural glycosaminoglycan product of HA synthases with diverse cosmetic and medical applications. While process and/or metabolic engineering are utilized for industrial HA production, the potential of protein engineering has barely... |
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Biochemical Characterization of β‐Amino Acid Incorporation in Fluvirucin B2 Biosynthesis
10.1002/cbic.201800169 2018-03-30 Naturally occurring lactams, such as the polyketide‐derived macrolactams, provide a diverse class of natural products that could enhance existing chemically produced lactams. While β‐amino acid loading in the fluvirucin B2 polyketide pathway has been proposed... |