|
Single-Cell Transcriptional Analysis
10.1146/annurev-anchem-061516-045228 2017-06-12 Despite being a relatively recent technological development, single-cell transcriptional analysis through high-throughput sequencing has already been used in hundreds of fruitful studies to make exciting new biological discoveries that would otherwise be chal... |
|
|
Bioanalytical Measurements Enabled by Surface-Enhanced Raman Scattering (SERS) Probes
10.1146/annurev-anchem-071015-041557 2017-06-12 Since its discovery in 1974, surface-enhanced Raman scattering (SERS) has gained momentum as an important tool in analytical chemistry. SERS is used widely for analysis of biological samples, ranging from in vitro cell culture models, to ex vivo tissue and bl... |
|
|
Applications of Surface Second Harmonic Generation in Biological Sensing
10.1146/annurev-anchem-071015-041453 2017-06-12 Surface second harmonic generation (SHG) is a coherent, nonlinear optical technique that is well suited for investigations of biomolecular interactions at interfaces. SHG is surface specific due to the intrinsic symmetry constraints on the nonlinear process, ... |
|
|
The Solution Assembly of Biological Molecules Using Ion Mobility Methods: From Amino Acids to Amyloid β-Protein
10.1146/annurev-anchem-071114-040304 2017-06-12 Ion mobility spectrometry-mass spectrometry (IMS-MS) methods are increasingly used to study noncovalent assemblies of peptides and proteins. This review focuses on the noncovalent self-assembly of amino acids and peptides, systems at the heart of the amyloid ... |
|
|
Single-Molecule Arrays for Protein and Nucleic Acid Analysis
10.1146/annurev-anchem-061516-045340 2017-06-12 The last few years have seen breakthroughs that will transform our ability to measure important analytes. Miniaturization of reaction volumes and confinement of analytes of interest into ultrasmall containers have greatly enhanced the sensitivity and throughp... |
|
|
Identification and Quantitation of Circulating Tumor Cells
10.1146/annurev-anchem-061516-045405 2017-06-12 Circulating tumor cells (CTCs) are shed from the primary tumor into the circulatory system and act as seeds that initiate cancer metastasis to distant sites. CTC enumeration has been shown to have a significant prognostic value as a surrogate marker in variou... |
|
|
Beyond Antibodies as Binding Partners: The Role of Antibody Mimetics in Bioanalysis
10.1146/annurev-anchem-061516-045205 2017-06-12 The emergence of novel binding proteins or antibody mimetics capable of binding to ligand analytes in a manner analogous to that of the antigen–antibody interaction has spurred increased interest in the biotechnology and bioanalytical communities. The goal is... |
|
|
Raman Imaging in Cell Membranes, Lipid-Rich Organelles, and Lipid Bilayers
10.1146/annurev-anchem-061516-045317 2017-06-12 Raman-based optical imaging is a promising analytical tool for noninvasive, label-free chemical imaging of lipid bilayers and cellular membranes. Imaging using spontaneous Raman scattering suffers from a low intensity that hinders its use in some cellular app... |
|
|
Analyzing the Heterogeneous Hierarchy of Cultural Heritage Materials: Analytical Imaging
10.1146/annurev-anchem-071015-041500 2017-06-12 Objects of cultural heritage significance are created using a wide variety of materials, or mixtures of materials, and often exhibit heterogeneity on multiple length scales. The effective study of these complex constructions thus requires the use of a suite o... |
|
|
Light-Addressable Potentiometric Sensors for Quantitative Spatial Imaging of Chemical Species
10.1146/annurev-anchem-061516-045158 2017-06-12 A light-addressable potentiometric sensor (LAPS) is a semiconductor-based chemical sensor, in which a measurement site on the sensing surface is defined by illumination. This light addressability can be applied to visualize the spatial distribution of pH or t... |