Latest Articles of Bioconjugate Chemistry


Design, Synthesis, and Enzymatic Evaluation of Novel ZnO Quantum Dot-Based Assay for Detection of Proteinase 3 Activity 10.1021/acs.bioconjchem.8b00100 2018-04-20 Herein, the synthesis and application of functionalized quantum dot-based protease probes is described. Such probes are composed of nontoxic ZnO nanocrystals decorated by amino groups followed by linker and labeled peptide attachment. Spherical NH2-terminated...
Development of a PEGylated-Based Platform for Efficient Delivery of Dietary Antioxidants Across the Blood–Brain Barrier 10.1021/acs.bioconjchem.8b00151 2018-04-19 The uptake and transport of dietary antioxidants remains the most important setback for their application in therapy. To overcome the limitations, a PEGylated-based platform was developed to improve the delivery properties of two dietary hydroxycinnamic (HCA)...
Protein Enables Conformation Transition of a Hydrogel Based on Pentapeptide and Boosts Immune Response in Vivo 10.1021/acs.bioconjchem.8b00044 2018-04-16 We report a supramolecular hydrogel based on dihistidine containing pentapeptides serving as a novel vaccine delivery system. Protein encapsulated into the hydrogel not only enhances the mechanical property up to 15-fold but also changes the conformation of t...
SpyCatcher-NTEV: A Circularly Permuted, Disordered SpyCatcher Variant for Less Trace Ligation 10.1021/acs.bioconjchem.8b00131 2018-04-16 The SpyTag/SpyCatcher reaction has emerged as a powerful way for bioconjugation, but it leaves a folded complex in the product after the formation of the isopeptide bond. To vary the location of the reactive residue and reduce the size of the complex and its ...
Novel 64Cu Labeled RGD2-BBN Heterotrimers for PET Imaging of Prostate Cancer 10.1021/acs.bioconjchem.8b00113 2018-04-13 Bombesin receptor 2 (BB2) and integrin αvβ3 receptor are privileged targets for molecular imaging of cancer because of their overexpression in a number of tumor tissues. The most recent developments in heterodimer-based radiopharmaceuticals concern BB2- and i...
Creation of Linear Carbon Dot Array with Improved Optical Properties through Controlled Covalent Conjugation with DNA 10.1021/acs.bioconjchem.8b00173 2018-04-12 Controlled conjugation of fluorescent carbon dots (CDs) with DNA and subsequent fabrication of the CDs into an array through hybridization mediated self-assembly in the solution phase is reported. Covalent conjugation of CD with DNA and the subsequent array f...
Protein Surface Structural Recognition in Inactive Areas: A New Immobilization Strategy for Acetylcholinesterase 10.1021/acs.bioconjchem.8b00160 2018-04-10 This work reported a new method of design for the immobilization of acetylcholinesterase (AChE) based on its molecular structure to improve its sensitivity and stability. The immobilization binding site on the surface of AChE was determined using MOLCAD’s mul...
Engineered Charge Redistribution of Gp2 Proteins through Guided Diversity for Improved PET Imaging of Epidermal Growth Factor Receptor 10.1021/acs.bioconjchem.8b00144 2018-04-05 The Gp2 domain is a protein scaffold for synthetic ligand engineering. However, the native protein function results in a heterogeneous distribution of charge on the conserved surface, which may hinder further development and utility. We aim to modulate charge...
CLICK CONJUGATION OF CLOAKED PEPTIDE LIGANDS TO MICROBUBBLES 10.1021/acs.bioconjchem.8b00084 2018-04-04 Interest in the use of targeted microbubbles for ultrasound molecular imaging (USMI) has been growing in recent years as a safe and efficacious means of diagnosing tumor angiogenesis and assessing response to therapy. Of particular interest are cloaked microb...
Comprehensive Biochemical and Biophysical Characterization of Hemoglobin-Based Oxygen Carrier Therapeutics: All HBOCs Are Not Created Equally 10.1021/acs.bioconjchem.8b00093 2018-04-02 The development of hemoglobin (Hb)-based oxygen carriers (HBOCs) has been hampered because of safety concerns in humans. Chemical and/or genetic modifications of the Hb introduce varied structural and conformational constraint on the molecule that resulted in...

Design, Synthesis, and Enzymatic Evaluation of Novel ZnO Quantum Dot-Based Assay for Detection of Proteinase 3 Activity

10.1021/acs.bioconjchem.8b00100

2018-04-20

Herein, the synthesis and application of functionalized quantum dot-based protease probes is described. Such probes are composed of nontoxic ZnO nanocrystals decorated by amino groups followed by linker and labeled peptide attachment. Spherical NH2-terminated...

Development of a PEGylated-Based Platform for Efficient Delivery of Dietary Antioxidants Across the Blood–Brain Barrier

10.1021/acs.bioconjchem.8b00151

2018-04-19

The uptake and transport of dietary antioxidants remains the most important setback for their application in therapy. To overcome the limitations, a PEGylated-based platform was developed to improve the delivery properties of two dietary hydroxycinnamic (HCA)...

Protein Enables Conformation Transition of a Hydrogel Based on Pentapeptide and Boosts Immune Response in Vivo

10.1021/acs.bioconjchem.8b00044

2018-04-16

We report a supramolecular hydrogel based on dihistidine containing pentapeptides serving as a novel vaccine delivery system. Protein encapsulated into the hydrogel not only enhances the mechanical property up to 15-fold but also changes the conformation of t...

SpyCatcher-NTEV: A Circularly Permuted, Disordered SpyCatcher Variant for Less Trace Ligation

10.1021/acs.bioconjchem.8b00131

2018-04-16

The SpyTag/SpyCatcher reaction has emerged as a powerful way for bioconjugation, but it leaves a folded complex in the product after the formation of the isopeptide bond. To vary the location of the reactive residue and reduce the size of the complex and its ...

Novel 64Cu Labeled RGD2-BBN Heterotrimers for PET Imaging of Prostate Cancer

10.1021/acs.bioconjchem.8b00113

2018-04-13

Bombesin receptor 2 (BB2) and integrin αvβ3 receptor are privileged targets for molecular imaging of cancer because of their overexpression in a number of tumor tissues. The most recent developments in heterodimer-based radiopharmaceuticals concern BB2- and i...

Creation of Linear Carbon Dot Array with Improved Optical Properties through Controlled Covalent Conjugation with DNA

10.1021/acs.bioconjchem.8b00173

2018-04-12

Controlled conjugation of fluorescent carbon dots (CDs) with DNA and subsequent fabrication of the CDs into an array through hybridization mediated self-assembly in the solution phase is reported. Covalent conjugation of CD with DNA and the subsequent array f...

Protein Surface Structural Recognition in Inactive Areas: A New Immobilization Strategy for Acetylcholinesterase

10.1021/acs.bioconjchem.8b00160

2018-04-10

This work reported a new method of design for the immobilization of acetylcholinesterase (AChE) based on its molecular structure to improve its sensitivity and stability. The immobilization binding site on the surface of AChE was determined using MOLCAD’s mul...

Engineered Charge Redistribution of Gp2 Proteins through Guided Diversity for Improved PET Imaging of Epidermal Growth Factor Receptor

10.1021/acs.bioconjchem.8b00144

2018-04-05

The Gp2 domain is a protein scaffold for synthetic ligand engineering. However, the native protein function results in a heterogeneous distribution of charge on the conserved surface, which may hinder further development and utility. We aim to modulate charge...

CLICK CONJUGATION OF CLOAKED PEPTIDE LIGANDS TO MICROBUBBLES

10.1021/acs.bioconjchem.8b00084

2018-04-04

Interest in the use of targeted microbubbles for ultrasound molecular imaging (USMI) has been growing in recent years as a safe and efficacious means of diagnosing tumor angiogenesis and assessing response to therapy. Of particular interest are cloaked microb...

Comprehensive Biochemical and Biophysical Characterization of Hemoglobin-Based Oxygen Carrier Therapeutics: All HBOCs Are Not Created Equally

10.1021/acs.bioconjchem.8b00093

2018-04-02

The development of hemoglobin (Hb)-based oxygen carriers (HBOCs) has been hampered because of safety concerns in humans. Chemical and/or genetic modifications of the Hb introduce varied structural and conformational constraint on the molecule that resulted in...