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A simple and ultrasensitive fluorescence assay for single-nucleotide polymorphism
10.1007/s00216-018-0874-4 2018-04-12 In this report, a simple, label-free and highly efficient nucleic acid amplification technique is developed for ultrasensitive detection of single-nucleotide polymorphism (SNP). Briefly, a designed padlock probe is first circularized by a DNA ligase when it p... |
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Proteomic approaches beyond expression profiling and PTM analysis
10.1007/s00216-018-1021-y 2018-04-10 Essentially, all cellular functions are executed by proteins. Different physiological and pathological conditions dynamically control various properties of proteins, including expression levels, post-translational modifications (PTMs), protein–protein interac... |
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Simultaneous determination of sulfur compounds from the sulfur pathway in rat plasma by liquid chromatography tandem mass spectrometry: application to the study of the effect of Shao Fu Zhu Yu decoction
10.1007/s00216-018-1038-2 2018-04-10 A sensitive, accurate, and time-saving approach was developed for the simultaneous quantification of eight sulfur compounds in the sulfur pathway, which could reflect the status of an organism, including oxidative stress, signal transduction, enzyme reaction,... |
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Characterization of bioactive compounds of Annona cherimola L. leaves using a combined approach based on HPLC-ESI-TOF-MS and NMR
10.1007/s00216-018-1051-5 2018-04-09 Annona cherimola Mill. (cherimoya) has widely been used as food crop. The leaves of this tree possess several health benefits, which are, in general, attributed mainly to its bioactive composition. However, literature concerning a comprehensive characterizati... |
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Fabrication of an immunosensor for early and ultrasensitive determination of human tissue plasminogen activator (tPA) in myocardial infraction and breast cancer patients
10.1007/s00216-018-1005-y 2018-04-07 Sensitive detection of biomarkers will mean accurate and early diagnosis of diseases. A tissue plasminogen activator (tPA) has a crucial role in many cardiovascular diseases and it is related to many processes such as angiogenesis in cancer cells. Therefore, ... |
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Identification and quantification of glue-like off-odors in elastic therapeutic tapes
10.1007/s00216-018-1046-2 2018-04-06 Elastic therapeutic tapes are an important tool in the field of physical therapy and medicine. These tapes contain types of adhesive. However, sensory evaluations revealed the release of pronounced and irritating odors of the tapes. Negative odors were, among... |
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Precise, accurate and user-independent blood collection system for dried blood spot sample preparation
10.1007/s00216-018-0993-y 2018-04-05 An accurate and precise 3 μL blood collection and dispensing system is presented for the preparation of dried blood spot (DBS) samples. Using end-to-end glass capillaries in conjugation with pre-punched DBS pads, a blood micro collection system was developed ... |
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Online low-field NMR spectroscopy for process control of an industrial lithiation reaction—automated data analysis
10.1007/s00216-018-1020-z 2018-04-04 Monitoring specific chemical properties is the key to chemical process control. Today, mainly optical online methods are applied, which require time- and cost-intensive calibration effort. NMR spectroscopy, with its advantage being a direct comparison method ... |
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Physicochemical study of natural fractionated biocolloid by asymmetric flow field-flow fractionation in tandem with various complementary techniques using biologically synthesized silver nanocomposites
10.1007/s00216-018-0967-0 2018-04-03 Asymmetric flow field-flow fractionation coupled with use of ultraviolet–visible, multiangle light scattering (MALLS), and dynamic light scattering (DLS) detectors was used for separation and characterization of biologically synthesized silver composites in t... |
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Silicone wristbands compared with traditional polycyclic aromatic hydrocarbon exposure assessment methods
10.1007/s00216-018-0992-z 2018-04-02 Currently there is a lack of inexpensive, easy-to-use technology to evaluate human exposure to environmental chemicals, including polycyclic aromatic hydrocarbons (PAHs). This is the first study in which silicone wristbands were deployed alongside two traditi... |