Latest Articles of Analytical Chemistry


Large-Scale Differentiation and Site Specific Discrimination of Hydroxyproline Isomers by Electron Transfer/Higher-Energy Collision Dissociation (EThcD) Mass Spectrometry 10.1021/acs.analchem.8b00413 2018-04-20 3- and 4-Hydroxyprolines (HyP) are regioisomers that play different roles in various species and organs. Despite their distinct functions inside cells, they are generally considered indistinguishable using mass spectrometry due to their identical masses. Here...
Spatial-Resolution Cell Type Proteome Profiling of Cancer Tissue by Fully Integrated Proteomics Technology 10.1021/acs.analchem.8b00596 2018-04-18 Increasing attention has been focused on cell type proteome profiling for understanding the heterogeneous multicellular microenvironment in tissue samples. However, current cell type proteome profiling methods need large amounts of starting materials which pr...
Determination of Osmium Concentration and Isotope Composition at Ultra-low Level in Polar Ice and Snow 10.1021/acs.analchem.8b00150 2018-04-18 Here we use two chemical separation procedures to determine exceptionally low Os concentrations (∼10–15 g g–1) and Os isotopic composition in polar snow/ice. Approximately 50 g of meltwater is spiked with 190Os tracer solution and frozen at −20 °C in quartz-g...
MoFi: A Software Tool for Annotating Glycoprotein Mass Spectra by Integrating Hybrid Data from the Intact Protein and Glycopeptide Level 10.1021/acs.analchem.8b00019 2018-04-18 Hybrid mass spectrometry (MS) is an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity. Since hybrid MS combines information from different experimental levels, it crucially depends on computational meth...
Determination of Collision Cross-Sections of Protein Ions in an Orbitrap Mass Analyzer 10.1021/acs.analchem.8b00724 2018-04-18 We demonstrate a method for determining the collision cross-sections (CCSs) of protein ions based on the decay rate of the time-domain transient signal from an Orbitrap mass analyzer. Multiply charged ions of ubiquitin, cytochrome c, and myoglobin were genera...
Exploration of Nanoparticle-Mediated Photothermal Effect of TMB-H2O2 Colorimetric System and Its Application in a Visual Quantitative Photothermal Immunoassay 10.1021/acs.analchem.8b00842 2018-04-17 The exploration of new physical and chemical properties of materials and their innovative application in different fields are of great importance to advance analytical chemistry, material science, and other important fields. Herein, we, for the first time, di...
Chemically Regulated ROS Generation from Gold Nanoparticles for Enzyme-Free Electrochemiluminescent Immunosensing 10.1021/acs.analchem.8b00118 2018-04-17 In the present work, we report on an enzyme-free electrochemiluminescent (ECL) immunosensing scheme utilizing the catalytic generation of reactive oxygen species (ROS) from gold nanoparticles (AuNPs) (diameter ≥5 nm) dispersed in aqueous solutions of trishydr...
Fabrication of a Microfluidic Flame Atomic Emission Spectrometer: a Flame-on-a-Chip 10.1021/acs.analchem.8b00774 2018-04-17 This work demonstrates for the first time the fabrication of a microfluidic flame atomic emission spectrometer (FAES), which incorporates a microburner and flame (flame-on-a-chip). An essential part of the device is a thermospray system applied for effective ...
Bioluminescence Imaging of Carbon Monoxide in Living Cells and Nude Mice Based on Pd0-Mediated Tsuji–Trost Reaction 10.1021/acs.analchem.8b01102 2018-04-17 Carbon monoxide (CO) is highly toxic and lethal to humans and animals because of its strong affinity for hemoglobin, while this “silent killer” is constantly generated in the body as a cell-signaling molecule of the gasotransmitter family in various pathologi...
Macroscopic X-ray Powder Diffraction Scanning, a New Method for Highly Selective Chemical Imaging of Works of Art: Instrument Optimization 10.1021/acs.analchem.8b00240 2018-04-17 In the past decade macroscopic X-ray fluorescence imaging (MA-XRF) has become established as a method for the noninvasive investigation of flat painted surfaces, yielding large scale elemental maps. MA-XRF is limited by a lack of specificity, only allowing fo...

Large-Scale Differentiation and Site Specific Discrimination of Hydroxyproline Isomers by Electron Transfer/Higher-Energy Collision Dissociation (EThcD) Mass Spectrometry

10.1021/acs.analchem.8b00413

2018-04-20

3- and 4-Hydroxyprolines (HyP) are regioisomers that play different roles in various species and organs. Despite their distinct functions inside cells, they are generally considered indistinguishable using mass spectrometry due to their identical masses. Here...

Spatial-Resolution Cell Type Proteome Profiling of Cancer Tissue by Fully Integrated Proteomics Technology

10.1021/acs.analchem.8b00596

2018-04-18

Increasing attention has been focused on cell type proteome profiling for understanding the heterogeneous multicellular microenvironment in tissue samples. However, current cell type proteome profiling methods need large amounts of starting materials which pr...

Determination of Osmium Concentration and Isotope Composition at Ultra-low Level in Polar Ice and Snow

10.1021/acs.analchem.8b00150

2018-04-18

Here we use two chemical separation procedures to determine exceptionally low Os concentrations (∼10–15 g g–1) and Os isotopic composition in polar snow/ice. Approximately 50 g of meltwater is spiked with 190Os tracer solution and frozen at −20 °C in quartz-g...

MoFi: A Software Tool for Annotating Glycoprotein Mass Spectra by Integrating Hybrid Data from the Intact Protein and Glycopeptide Level

10.1021/acs.analchem.8b00019

2018-04-18

Hybrid mass spectrometry (MS) is an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity. Since hybrid MS combines information from different experimental levels, it crucially depends on computational meth...

Determination of Collision Cross-Sections of Protein Ions in an Orbitrap Mass Analyzer

10.1021/acs.analchem.8b00724

2018-04-18

We demonstrate a method for determining the collision cross-sections (CCSs) of protein ions based on the decay rate of the time-domain transient signal from an Orbitrap mass analyzer. Multiply charged ions of ubiquitin, cytochrome c, and myoglobin were genera...

Exploration of Nanoparticle-Mediated Photothermal Effect of TMB-H2O2 Colorimetric System and Its Application in a Visual Quantitative Photothermal Immunoassay

10.1021/acs.analchem.8b00842

2018-04-17

The exploration of new physical and chemical properties of materials and their innovative application in different fields are of great importance to advance analytical chemistry, material science, and other important fields. Herein, we, for the first time, di...

Chemically Regulated ROS Generation from Gold Nanoparticles for Enzyme-Free Electrochemiluminescent Immunosensing

10.1021/acs.analchem.8b00118

2018-04-17

In the present work, we report on an enzyme-free electrochemiluminescent (ECL) immunosensing scheme utilizing the catalytic generation of reactive oxygen species (ROS) from gold nanoparticles (AuNPs) (diameter ≥5 nm) dispersed in aqueous solutions of trishydr...

Fabrication of a Microfluidic Flame Atomic Emission Spectrometer: a Flame-on-a-Chip

10.1021/acs.analchem.8b00774

2018-04-17

This work demonstrates for the first time the fabrication of a microfluidic flame atomic emission spectrometer (FAES), which incorporates a microburner and flame (flame-on-a-chip). An essential part of the device is a thermospray system applied for effective ...

Bioluminescence Imaging of Carbon Monoxide in Living Cells and Nude Mice Based on Pd0-Mediated Tsuji–Trost Reaction

10.1021/acs.analchem.8b01102

2018-04-17

Carbon monoxide (CO) is highly toxic and lethal to humans and animals because of its strong affinity for hemoglobin, while this “silent killer” is constantly generated in the body as a cell-signaling molecule of the gasotransmitter family in various pathologi...

Macroscopic X-ray Powder Diffraction Scanning, a New Method for Highly Selective Chemical Imaging of Works of Art: Instrument Optimization

10.1021/acs.analchem.8b00240

2018-04-17

In the past decade macroscopic X-ray fluorescence imaging (MA-XRF) has become established as a method for the noninvasive investigation of flat painted surfaces, yielding large scale elemental maps. MA-XRF is limited by a lack of specificity, only allowing fo...