Interaction of digestive enzymes with tunable light emitting quantum dots: a thorough Spectroscopic investigation.

Vaishnavi Ellappan, Manibalan Kesavan, Parameshwari Ramalingam, Jeganathan Kulandaivel, Renganathan Rajalingam

Index: Luminescence 30 , 978-89, (2015)

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Abstract

In this article, we have examined the direct spectroscopic and microscopic evidence of efficient quantum dots-α-chymotrypsin (ChT) interaction. The intrinsic fluorescence of digestive enzyme is reduced in the presence of quantum dots through ground-state complex formation. Based on the fluorescence data, quenching rate constant, binding constant, and number of binding sites are calculated under optimized experimental conditions. Interestingly, fluorescence quenching method clearly illustrated the size dependent interaction of MPA-CdTe quantum dots. Conformational change of ChT was traced using synchronous fluorescence measurements, circular dichroism and FTIR spectroscopic methods. Furthermore, the AFM results revealed that the individual enzyme molecule dimensions were changed after interacting with quantum dot. Consequently, this result could be helpful for constructing safe and effective utilisation of QDs in biological applications.Copyright © 2015 John Wiley & Sons, Ltd.