Simultaneous determination of danshensu, salvianolic acid B, and paeonol in ShuangDan oral liquid by HPLC.
Hua Li, Siwang Wang, Yanhua Xie, Bangle Zhang, Jianbo Wang, Qian Yang, Wei Cao
Index: J. AOAC Int. 96(1) , 20-3, (2013)
Full Text: HTML
Abstract
An HPLC-photodiode array detector (PDAD) method was developed for simultaneous determination of danshensu, salvianolic acid B, and paeonol in a traditional Chinese medicinal preparation ShuangDan oral liquid, which is widely used to treat coronary heart disease in China. The samples were separated on a SinoChrom ODS-BP C18 column with the column temperature maintained at 30 degrees C. The mobile phase was composed of methanol and 2% (v/v) glacial acetic acid at a flow rate of 1.0 mL/min. The separation was finished within 30 min using linear gradient elution. All calibration curves showed good linearity (r > 0.9997) within the test ranges. The method was simple, reliable, accurate, and specific. The intraday and interday precisions (RSD) were less than 0.85%. The recoveries were between 96.97 and 102.14%. The method was successfully applied to the determination of the three components in three commercial samples of ShuangDan oral liquid. The results indicated that the developed HPLC-PDAD assay could be readily utilized as a comprehensive quality control method for ShuangDan oral liquid.
Related Compounds
Related Articles:
2012-11-15
[Int. J. Pharm. 438(1-2) , 91-7, (2012)]
2012-01-01
[Biol. Pharm. Bull. 35(5) , 767-72, (2012)]
2012-01-01
[Planta Med. 78(2) , 135-40, (2012)]
Effect of paeonol on antioxidant and immune regulatory activity in hepatocellular carcinoma rats.
2012-01-01
[Molecules 17(4) , 4672-83, (2012)]
2012-01-01
[PLoS ONE 7(11) , e48872, (2012)]