Induction of TGF-_ and IL-10 production in dendritic cells using astilbin to inhibit dextran sulfate sodium-induced colitis.

Yanbing Ding, Yu Liang, Bin Deng, Ahui Qiao, Keyan Wu, Weiming Xiao, Weijuan Gong

Index: Biochem. Biophys. Res. Commun. 446(2) , 529-34, (2014)

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Abstract

Astilbin, a major bioactive compound from Rhizoma smilacis glabrae, has been reported to possess anti-inflammatory properties. Our study first evaluated astilbin on dextran sulfate sodium (DSS)-induced acute colitis in mice. By intraperitoneal injection of astilbin, the severity of colitis was attenuated, and the serum levels of IL-10 and TGF-_ were increased. Using flow cytometry, a higher number of IL-10(+) dendritic cells (DCs) and TGF-_(+) DCs and a lower number of CD86(+) DCs, IL-12 p40(+) DCs, and IL-1_(+) DCs were detected in the spleen of mice with colitis after astilbin treatment. The administration of astilbin also resulted in the upregulation of CD103(+) expression in colonic DCs. In a coculture system, murine bone marrow-derived DCs pretreated with astilbin resulted in an enhanced production of CD4(+)CD25(+)Foxp3(+) T cells. The results of this study show that astilbin could be a candidate drug for inflammatory bowel disease by mediating the regulatory functions of DCs.