Carbohydrate Research 2002-06-05

Efficient chemoenzymatic synthesis of globotriose and its derivatives with a recombinant alpha-(1-->4)-galactosyltransferase.

Jianbo Zhang, Przemyslaw Kowal, Jianwen Fang, Peter Andreana, Peng George Wang

Index: Carbohydr. Res. 337(11) , 969-76, (2002)

Full Text: HTML

Abstract

A truncated alpha-(1-->4)-galactosyltransferase (LgtC) gene from Neisseria meningitidis was cloned. The recombinant glycosyltransferase was expressed in Escherichia coli BL21 (DE3) strain with high specific activity (5 units/mg protein). Its acceptor specificity was carefully characterized. Then the purified enzyme was utilized in highly efficient syntheses of globotriose and a variety of alpha-(1-->4)-galactosylated derivatives as potential antibacterial agents.

Related Compounds

Related Articles:

Carbosilane dendrimers bearing globotriaoses: syntheses of globotrioasyl derivative and introduction into carbosilane dendrimers.

2006-08-01

[Biomacromolecules 7(8) , 2274-83, (2006)]

Structural analysis of the interaction between Shiga toxin B subunits and linear polymers bearing clustered globotriose residues.

2006-03-01

[Infect. Immun. 74(3) , 1984-8, (2006)]

Determination of the cell adhesion specificity of Streptococcus suis with the complete set of monodeoxy analogues of globotriose.

1999-01-01

[Glycoconj. J. 16(1) , 67-71, (1999)]

Structure of extended lipopolysaccharide glycoforms containing two globotriose units in Haemophilus influenzae serotype b strain RM7004.

2003-04-22

[Biochemistry 42(15) , 4463-75, (2003)]

In vitro and in vivo effects of soluble, monovalent globotriose on bacterial attachment and colonization.

2005-09-01

[Antimicrob. Agents Chemother. 49(9) , 3842-6, (2005)]

More Articles...