Green and sensitive supercritical fluid chromatographic-tandem mass spectrometric method for the separation and determination of flutriafol enantiomers in vegetables, fruits, and soil.

Yan Tao, Fengshou Dong, Jun Xu, Xingang Liu, Youpu Cheng, Na Liu, Zenglong Chen, Yongquan Zheng

Index: J. Agric. Food Chem. 62(47) , 11457-64, (2014)

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Abstract

A green and sensitive chiral analytical method was developed to determine flutriafol enantiomers in vegetables (tomato, cucumber), fruits (apple, grape), and soil by supercritical fluid chromatography-tandem mass spectrometry. The enantioseparation was performed within 3.50 min using Chiralpak IA-3 column with CO2/methanol (88:12, v/v) as the mobile phase at a 2.2 mL/min flow rate. The postcolumn compensation technology provided with 1% formic acid/methanol greatly improved the ionization efficiency of mass spectrometry. Column temperature, auto back pressure regulator pressure, and flow rate of compensation solvent were optimized to 30 °C, 2200 psi, and 0.1 mL/min, respectively. The simple and fast QuEChERS pretreatment method was adopted. Mean recoveries for flutriafol enantiomers were 77.2-98.9% with RSDs ≤ 9.6% in all matrices. The limits of quantification ranged from 0.41 to 1.18 μg/kg. Well-applied to analyze authentic samples, the developed method could act as a versatile strategy for the analysis of flutriafol enantiomers in food and environmental matrices.