Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots.

Daniel Gonzalez, Chiara Melloni, Brenda B Poindexter, Ram Yogev, Andrew M Atz, Janice E Sullivan, Susan R Mendley, Paula Delmore, Amy Delinsky, Kanecia Zimmerman, Andrew Lewandowski, Barrie Harper, Kenneth C Lewis, Daniel K Benjamin, Michael Cohen-Wolkowiez

Index: Bioanalysis 7 , 1137-49, (2015)

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Abstract

Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices.For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices.A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.