Ultraperformance liquid chromatography with electrospray ionization ion trap mass spectrometry for chondroitin disaccharide analysis.
Kemal Solakyildirim, Zhenqing Zhang, Robert J Linhardt
Index: Anal. Biochem. 397(1) , 24-8, (2010)
Full Text: HTML
Abstract
Chondroitin sulfate (CS) has an important role in cell division, in the central nervous system, and in joint-related pathologies such as osteoarthritis. Due to the complex chemical structure and biological importance of CS, simple, sensitive, high resolution, and robust analytical methods are needed for the analysis of CS disaccharides and oligosaccharides. An ion-pairing, reversed-phase, ultraperformance liquid chromatography (IPRP-UPLC) separation, coupled to electrospray ionization mass spectrometry with an ion trap mass analyzer, was applied for the analyses of CS-derived disaccharides. UPLC separation technology uses small particle diameter, short column length, and elevated column temperature to obtain high resolution and sensitivity. Hexylamine (15 mM) was selected as the optimal ion-pairing reagent.Copyright 2009 Elsevier Inc. All rights reserved.
Related Compounds
Related Articles:
Plasmonic ag@oxide nanoprisms for enhanced performance of organic solar cells.
2015-05-01
[Small 11 , 2454-62, (2015)]
2015-01-28
[Phys. Chem. Chem. Phys. 17(4) , 2357-65, (2014)]
2014-01-01
[Eur. J. Mass Spectrom. (Chichester, Eng.) 20(6) , 467-75, (2015)]
Mosquito odorant receptor for DEET and methyl jasmonate.
2014-11-18
[Proc. Natl. Acad. Sci. U. S. A. 111(46) , 16592-7, (2014)]
Unexpected photoluminescence of fluorinated naphthalene diimides.
2015-03-02
[Chemistry 21(10) , 4133-40, (2015)]